Nuclear Antigen 抗体
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- 抗原
- Nuclear Antigen
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适用
- 人, 大鼠, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 产品特性
- This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Human cell nuclei were used as the immunogen for the Nuclear Antigen antibody.
- 克隆位点
- NUCM1-1
- 亚型
- IgG1 kappa
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- 应用备注
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Titering of the Nuclear Antigen antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH 6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Prediluted IHC only format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, *For IHC use only*
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Nuclear Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Nuclear Antigen
- 背景
- This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.
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