Histone 3 抗体 (H3K27me)
(14 references)-
- 抗原 See all Histone 3 (H3) 抗体
- Histone 3 (H3)
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抗原表位
- H3K27me
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Histone 3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq)
- 交叉反应
- 人, 小鼠, 大鼠
- 产品特性
- Methylated Antibodies
- 纯化方法
- Affinity purification
- 免疫原
- A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
- 亚型
- IgG
- Top Product
- Discover our top product H3 Primary Antibody
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anti-Histone 3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27ac) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (3meLys4) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me3) antibodyH3 适用: 人, 小鼠 WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 小鼠 Monoclonal MABI 0333 unconjugated
anti-Histone 3 (H3) (H3K9ac) antibodyH3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (AA 71-136) antibodyH3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (C-Term) antibodyH3 适用: 人 WB, IHC, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 适用: 人 WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K79me) antibodyH3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200,IP,1:50 - 1:200,ChIP,1:20 - 1:100,ChIP-seq,1:20 - 1:100
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid freeze / thaw cycles
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. Avoid freeze / thaw cycles.
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: "Evolutionary dynamics of 3D genome architecture following polyploidization in cotton." in: Nature plants, Vol. 4, Issue 2, pp. 90-97, (2018) (PubMed).
: "WOX11 recruits a histone H3K27me3 demethylase to promote gene expression during shoot development in rice." in: Nucleic acids research, Vol. 46, Issue 5, pp. 2356-2369, (2018) (PubMed).
: "In vivo CRISPR screening unveils histone demethylase UTX as an important epigenetic regulator in lung tumorigenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 17, pp. E3978-E3986, (2018) (PubMed).
: "Estrogen, through estrogen receptor 1, regulates histone modifications and chromatin remodeling during spermatogenesis in adult rats." in: Epigenetics, Vol. 12, Issue 11, pp. 953-963, (2018) (PubMed).
: "Ascorbic acid induces global epigenetic reprogramming to promote meiotic maturation and developmental competence of porcine oocytes." in: Scientific reports, Vol. 8, Issue 1, pp. 6132, (2018) (PubMed).
: "DMBA acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes." in: Scientific reports, Vol. 7, Issue 1, pp. 1687, (2018) (PubMed).
: "Metformin inhibits ovarian cancer via decreasing H3K27 trimethylation." in: International journal of oncology, Vol. 52, Issue 6, pp. 1899-1911, (2018) (PubMed).
: "Iron chelation inhibits cancer cell growth and modulates global histone methylation status in colorectal cancer." in: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, Vol. 31, Issue 5, pp. 797-805, (2018) (PubMed).
: "Asymmetric subgenome selection and cis-regulatory divergence during cotton domestication." in: Nature genetics, Vol. 49, Issue 4, pp. 579-587, (2017) (PubMed).
: "Cooperation between the H3K27me3 Chromatin Mark and Non-CG Methylation in Epigenetic Regulation." in: Plant physiology, Vol. 172, Issue 2, pp. 1131-1141, (2017) (PubMed).
: "Direct regulation of genes involved in sperm release by estrogen and androgen through their receptors and coregulators." in: The Journal of steroid biochemistry and molecular biology, Vol. 171, pp. 66-74, (2017) (PubMed).
: "The EZH1-SUZ12 complex positively regulates the transcription of NF-κB target genes through interaction with UXT." in: Journal of cell science, Vol. 129, Issue 12, pp. 2343-53, (2017) (PubMed).
: "EZH2-mediated epigenetic silencing of TIMP2 promotes ovarian cancer migration and invasion." in: Scientific reports, Vol. 7, Issue 1, pp. 3568, (2017) (PubMed).
: "Regulation of histone methylation and reprogramming of gene expression in the rice inflorescence meristem." in: The Plant cell, Vol. 27, Issue 5, pp. 1428-44, (2016) (PubMed).
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: "Evolutionary dynamics of 3D genome architecture following polyploidization in cotton." in: Nature plants, Vol. 4, Issue 2, pp. 90-97, (2018) (PubMed).
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- 抗原
- Histone 3 (H3)
- 别名
- Histone H3 (H3 产品)
- 别名
- H-3 antibody, histocompatibility 3 antibody, H3 antibody
- 背景
- Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling.,H3.4,H3/g,H3FT,H3t,HIST3H3,Histone H3,HIST1H3A,Signal Transduction,MAPK-Erk Signaling Pathway,MAPK-P38 Signaling Pathway,Epigenetics & Nuclear Signaling,Epigenetic Modifications,Methylation,Histone H3
- 分子量
- 15 kDa
- 基因ID
- 8290
- UniProt
- Q16695
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