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WT1 抗体 (AA 1-302)

Name: WT1 抗体 (AA 1-302)
Brand: antibodies-online
SKU: ABIN3021698
Availability: OutOfStock

WT1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry (IHC) image for anti-Wilms Tumor 1 (WT1) (AA 1-302) antibody (ABIN3021698) (WT1 抗体 (AA 1-302))

IHC

Western blot analysis of extracts of various cell lines, using WT1 antibody (ABIN3021697, ABIN3021698, ABIN3021699, ABIN1513692 and ABIN6215537) at 1:1000 dilution. (WT1 抗体 (AA 1-302))

Immunofluorescence analysis of HeLa cell using WT1 antibody. (WT1 抗体 (AA 1-302))

Western blot analysis of extracts of various cell lines, using WT1 antibody. (WT1 抗体 (AA 1-302))

The expression levels of podocyte injury makers including WT1, Nephrin and Desmin in the MPC5 cells in five groups were quantified by western blot and the internal reference was GAPDH. (WT1 抗体 (AA 1-302))

5 images

(1 reference)

产品编号 ABIN3021698

发货至: 中国

Datasheet as PDF

抗原 See all WT1 抗体

WT1 (Wilms Tumor 1 (WT1))
抗原表位
32

16

15

6

6

6

5

5

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 1-302
适用
144

67

60

17

4

4

3

2

2

1

1

1

1
人
宿主
81

62

1
兔
Compatible Secondaries
克隆类型
86

58
多克隆
标记
70

10

5

5

5

5

5

5

5

4

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This WT1 antibody is un-conjugated
应用范围
87

81

59

56

32

22

20

14

13

5

5

4

3

2

1

1

1
Western Blotting (WB)

序列

MEKGYSTVTF DGTPSYGHTP SHHAAQFPNH SFKHEDPMGQ QGSLGEQQYS VPPPVYGCHT PTDSCTGSQA LLLRTPYSSD NLYQMTSQLE CMTWNQMNLG ATLKGVAAGS SSSVKWTEGQ SNHSTGYESD NHTTPILCGA QYRIHTHGVF RGIQDVRRVP GVAPTLVRSA SETSEKRPFM CAYPGCNKRY FKLSHLQMHS RKHTGEKPYQ CDFKDCERRF SRSDQLKRHQ RRHTGVKPFQ CKTCQRKFSR SDHLKTHTRT HTGEKPFSCR WPSCQKKFAR SDELVRHHNM HQRNMTKLQL AL

交叉反应

人, 小鼠, 大鼠

产品特性

Polyclonal Antibodies

免疫原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human WT1 (NP_001185480.1).

亚型

IgG
anti-Wilms Tumor 1 (WT1) (AA 1-181) antibody
WT1 适用: 人 FACS, IHC, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 7F9E8 unconjugated

anti-Wilms Tumor 1 (WT1) antibody
WT1 适用: 人, 小鼠, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal rWT1-857 unconjugated Recombinant Antibody

anti-Wilms Tumor 1 (WT1) (AA 1-181) antibody
WT1 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 7F9G7H2 unconjugated

anti-Wilms Tumor 1 (WT1) (AA 1-181) antibody
WT1 适用: 人, 小鼠, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal 6F-H2 unconjugated

anti-Wilms Tumor 1 (WT1) (C-Term) antibody
WT1 适用: 人, 小鼠, 大鼠, 犬, 兔, Bat, 小鸡, 猴, 非洲爪蟾 IHC, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Wilms Tumor 1 (WT1) (AA 1-302) antibody
WT1 适用: 人 IF, WB 宿主: 兔 Polyclonal unconjugated

anti-Wilms Tumor 1 (WT1) (AA 314-479) antibody
WT1 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 5G11A5 unconjugated

anti-Wilms Tumor 1 (WT1) (AA 346-375) antibody
WT1 适用: 人, 小鼠 IF, FACS, WB, IHC (p) 宿主: 兔 Polyclonal RB18841 unconjugated

anti-Wilms Tumor 1 (WT1) (Middle Region) antibody
WT1 适用: 人, 小鼠, 大鼠, Cow, 犬, 兔, Pig, 斑马鱼, 马 IHC, WB 宿主: 兔 Polyclonal unconjugated

anti-Wilms Tumor 1 (WT1) (AA 346-375) antibody
WT1 适用: 人 IF, FACS, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

WB,1:500 - 1:2000

限制

仅限研究用
状态

Liquid

缓冲液

PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze / thaw cycles

储存条件

-20 °C

储存方法

Store at -20°C. Avoid freeze / thaw cycles.
Wang, Luo, Du, Shu, Guo, Li: "Nuclear paraspeckle assembly transcript 1 promotes the podocyte injury via targeting miR-23b-3p/B-cell lymphoma-2 interacting protein 3 like axis." in: Renal failure, Vol. 44, Issue 1, pp. 1961-1975, (2022) (PubMed).
抗原

WT1 (Wilms Tumor 1 (WT1))

别名

WT1 (WT1 产品)

别名

AWT1 antibody, EWS-WT1 antibody, GUD antibody, NPHS4 antibody, WAGR antibody, WIT-2 antibody, WT33 antibody, CWT1 antibody, WT1 antibody, XWT1a antibody, wt-1 antibody, wt1 antibody, xWT1 antibody, WT-1 antibody, gud antibody, wagr antibody, wt33 antibody, xwt1 antibody, wit-2 antibody, si:ch211-160j6.3 antibody, zgc:152697 antibody, D630046I19Rik antibody, Wt-1 antibody, XWT1b antibody, XeWT1 antibody, Wilms tumor 1 antibody, Wilms tumor 1 S homeolog antibody, wilms tumor 1b antibody, Wilms tumor protein antibody, Wilms tumor 1 homolog antibody, Wilms tumor 1 L homeolog antibody, WT1 antibody, wt1.S antibody, wt1 antibody, wt1b antibody, Wt1 antibody, wt1.L antibody

背景

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.,WT1,AWT1,EWS-WT1,GUD,NPHS4,WAGR,WIT-2,WT33,Epigenetics & Nuclear Signaling,Transcription Factors,Cancer,Tumor biomarkers,Signal Transduction,ErbB-HER Signaling Pathway,Cell Biology & Developmental Biology,Apoptosis,WT1

分子量

33 kDa/34 kDa/47 kDa/48 kDa/49 kDa/55 kDa/56 kDa

基因ID

7490

UniProt

P19544

途径

Tube Formation