FZD2 抗体 (Internal Region)
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- 抗原 See all FZD2 抗体
- FZD2 (Frizzled Family Receptor 2 (FZD2))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This FZD2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)
- 原理
- Frizzled 2
- 序列
- RYATLEHPFH CPR
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1.
- 亚型
- IgG
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- 应用备注
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Immunohistochemistry: In paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles. Recommended concentration: 2-5 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx 60-65 kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24 kDa was also observed in some lysates which was successfully blocked by incubation with
Peptide ELISA: antibody detection limit dilution 1:64000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Immunoreactivity of Wnt5a, Fzd2, Fzd6, and Ryk in glioblastoma: evaluative methodology for DAB chromogenic immunostaining." in: Brain tumor pathology, Vol. 31, Issue 2, pp. 85-93, (2014) (PubMed).
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Immunoreactivity of Wnt5a, Fzd2, Fzd6, and Ryk in glioblastoma: evaluative methodology for DAB chromogenic immunostaining." in: Brain tumor pathology, Vol. 31, Issue 2, pp. 85-93, (2014) (PubMed).
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- 抗原
- FZD2 (Frizzled Family Receptor 2 (FZD2))
- 别名
- FZD2 (FZD2 产品)
- 别名
- BG01711 antibody, CG9739 antibody, D-Fz2 antibody, D-fz2 antibody, DFZ2 antibody, DFz-2 antibody, DFz2 antibody, Dfrz2 antibody, Dfz2 antibody, Dfz[[2]] antibody, Dm Fz2 antibody, Dmel\\CG9739 antibody, FZ2 antibody, Fz2 antibody, dFz2 antibody, dfz(2) antibody, dfz2 antibody, dfz2r antibody, frz2 antibody, fz-2 antibody, fz8 antibody, zfz2 antibody, zg08 antibody, cb383 antibody, wu:fb70g09 antibody, FZD2 antibody, fzE2 antibody, hFz2 antibody, Fz-10 antibody, Fzd10 antibody, cFz-2 antibody, frizzled2 antibody, fz2 antibody, xfz2 antibody, AL033370 antibody, AW456835 antibody, Fz10 antibody, Mfz10 antibody, Mfz10a antibody, frizzled 2 antibody, frizzled class receptor 2 antibody, frizzled class receptor 2 S homeolog antibody, fz2 antibody, fzd2 antibody, FZD2 antibody, Fzd2 antibody, fzd2.S antibody
- 背景
- FZD2 , frizzled homolog 2 (Drosophila) , frizzled 2
- 基因ID
- 2535, 57265, 64512
- NCBI登录号
- NP_001457
- 途径
- WNT signaling
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