Keratin 10/13 抗体
(3 references)-
- 抗原
- Keratin 10/13
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适用
- 人, Cat
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- The mouse monoclonal antibody DE-K13 reacts with cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues. DE-K13 recognizes only cytokeratin 13 on formalin-fixed, paraffin-embedded tissue sections.
- 交叉反应 （详细）
- Human, Feline (Cat), Other species Not tested
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Cytoskeletal preparation extracted from human ectocervical epithelium.
- 克隆位点
- DE-K13
- 亚型
- IgG2a
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anti-Keratin 10/13 antibody
适用: 人, Cat, 斑马鱼 WB, FACS, IHC (p), IHC, IHC (fro), ICC 宿主: 小鼠 Monoclonal DE-K13 unconjugated
anti-Keratin 10/13 antibody适用: 人, Cat WB, FACS, IHC (p), IHC, IHC (fro), ICC 宿主: 小鼠 Monoclonal DE-K13 unconjugated
anti-Keratin 10/13 antibody适用: 人, Cat WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal DE-K13 unconjugated
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- 应用备注
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Immunohistochemistry (paraffin sections): Standard ABC technique (DAB+), recommended dilution: 10 μg/mL (1:100), positive tissue: tonsil. Pretreatment: 0.1 % pepsin in 0.1 M HCl for 30 min at room temperature or Target Retrieval Solution - High pH . The antibody DE-K13 reacts exclusively with Cytokeratin 13.
Immunohistochemistry (frozen sections): Use at an assay dependent dilution.
Western blotting: recommended dilution: 1-3 μg/mL. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "Immunohistochemical analysis of cytokeratin expression in dog skin." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 63, Issue 1, pp. 1-4, (2001) (PubMed).
: "Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas." in: American journal of veterinary research, Vol. 54, Issue 7, pp. 1095-102, (1993) (PubMed).
: "Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells." in: American journal of veterinary research, Vol. 53, Issue 3, pp. 304-14, (1992) (PubMed).
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: "Immunohistochemical analysis of cytokeratin expression in dog skin." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 63, Issue 1, pp. 1-4, (2001) (PubMed).
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- 抗原
- Keratin 10/13
- 别名
- Cytokeratin 10+13
- 背景
- Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 10 and 13 both belong to type I family (acidic cytokeratins).,K10, K13, CK10, CK13, CK-10, CD13, CYK10, CYK13, KRT10, KRT13
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