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LAT 抗体 (Cytoplasmic Domain)

Name: LAT 抗体 (Cytoplasmic Domain)
Brand: antibodies-online
SKU: ABIN302069
Availability: OutOfStock

LAT 适用: 人 WB, IP, FACS 宿主: 小鼠 Monoclonal LAT-01 unconjugated

Western blotting analysis of human LAT using mouse monoclonal antibody LAT-01 in lysates of Jurkat cells (positive) and Raji cells (negative control) under non-reducing and reducing conditions. (LAT 抗体 (Cytoplasmic Domain))

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-LAT (LAT-01) purified antibody (concentration in sample 1 μg/mL, GAM APC). (LAT 抗体 (Cytoplasmic Domain))

FACS

Flow Cytometry analysis of LAT expression in peripheral blood lymphocytes using anti-human LAT (LAT-01) (surface markers were stained prior to intracellular staining of human LAT). (LAT 抗体 (Cytoplasmic Domain))

FACS

Separation of human CD3 positive LAT positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-LAT (LAT-01) purified antibody (concentration in sample 1 μg/mL, GAM APC). (LAT 抗体 (Cytoplasmic Domain))

FACS

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-LAT (LAT-01) purified antibody (concentration in sample 1 μg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue antibody (20 μL reagent / 100 μL of peripheral whole blood). (LAT 抗体 (Cytoplasmic Domain))

FACS

5 images

(2 references)

产品编号 ABIN302069

发货至: 中国

Datasheet as PDF

抗原 See all LAT 抗体

LAT (Linker For Activation of T Cells (LAT))
抗原表位
21

19

15

8

8

7

7

4

4

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cytoplasmic Domain
适用
133

60

43

1
人
宿主
126

8

2

1
小鼠
Compatible Secondaries
克隆类型
129

8
单克隆
标记
67

6

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3

3
This LAT antibody is un-conjugated
应用范围
75

46

39

39

16

13

9

9

4

2

1

1
Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

特异性

The antibody LAT-01 reacts with an intracellular epitope of LAT, a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.

无交叉反应

小鼠

交叉反应（详细）

Human

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT.

克隆位点

LAT-01

亚型

IgG1

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LAT 适用: 人 WB, ELISA, IHC, IP, IF 宿主: 兔 Monoclonal 2E4 unconjugated Recombinant Antibody

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anti-Linker For Activation of T Cells (LAT) (AA 30-237) antibody
LAT 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (pTyr161) antibody
LAT 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (Cytoplasmic Domain) antibody
LAT 适用: 人 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal LAT1111 unconjugated

anti-Linker For Activation of T Cells (LAT) (AA 25-221) antibody
LAT 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (AA 33-227) antibody
LAT 适用: 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (Tyr255) antibody
LAT 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (AA 1-233) antibody
LAT 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

应用备注

Immunoprecipitation: Tyrosine-phosphorylated form of LAT is not optimally recognized.
Flow cytometry: Recommended dilution: 1-4 μg/mL, intracellular staining. Tyrosine-phosphorylated form of LAT is not optimally recognized.
Western blotting: Recommended dilution: 1-2 μg/mL.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).

Tedoldi, Paterson, Hansmann, Natkunam, Rüdiger, Angelisova, Du, Roberton, Roncador, Sanchez, Pozzobon, Masir, Barry, Pileri, Mason, Marafioti, Horejsí: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." in: Blood, Vol. 107, Issue 1, pp. 213-21, (2005) (PubMed).
抗原

LAT (Linker For Activation of T Cells (LAT))

别名

LAT (LAT 产品)

别名

LAT antibody, lat antibody, LAT1 antibody, pp36 antibody, linker for activation of T-cells antibody, linker for activation of T cells antibody, LAT antibody, lat antibody, Lat antibody

背景

Linker for activation of T cells,LAT (linker for activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.,IMD52

基因ID

27040

UniProt

O43561

途径

TCR Signaling, Fc-epsilon Receptor Signaling Pathway