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GFP 抗体

GFP 适用: Aequorea victoria WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN302066
发货至: 中国
  • 抗原 See all GFP 抗体
    GFP (Green Fluorescent Protein (GFP))
    适用
    • 175
    • 15
    • 11
    • 9
    Aequorea victoria
    宿主
    • 64
    • 61
    • 35
    • 15
    • 9
    • 5
    • 1
    • 1
    克隆类型
    • 114
    • 72
    多克隆
    标记
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    This GFP antibody is un-conjugated
    应用范围
    • 149
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    • 25
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    Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    特异性
    The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in all species.
    交叉反应 (详细)
    Recognizes fusion proteins in all species
    纯化方法
    Purified from rabbit serum by affinity chromatography
    纯度
    > 95 % (by SDS-PAGE)
    免疫原
    EGFP, a native full-length protein
    Top Product
    Discover our top product GFP Primary Antibody
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
  • Porrero, Rubio-Garrido, Avendauno, Clascua: "Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice." in: Brain research, (2010) (PubMed).

    Valenta, Lukas, Doubravska, Fafilek, Korinek: "HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies." in: The EMBO journal, Vol. 25, Issue 11, pp. 2326-37, (2006) (PubMed).

  • 抗原
    GFP (Green Fluorescent Protein (GFP))
    别名
    GFP (GFP 产品)
    别名
    green fluorescent protein antibody, gfp antibody
    背景
    Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (λ,=509 nm) when excited (excitation by Blue or UV light, absorption peak λ,=395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chomophore mutations have been created. The EnhancedGFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.
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