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GFP 抗体 (AA 1-100)

Name: GFP 抗体 (AA 1-100)
Brand: antibodies-online
SKU: ABIN3020573
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, IF 宿主: 小鼠 Monoclonal unconjugated

Western blot analysis of over-expressed GFP fusion protein in 293 cell using GFP-Tag antibody. (GFP 抗体 (AA 1-100))

Immunofluorescence analysis of 293T cells using Mouse anti GFP-Tag mAb. (GFP 抗体 (AA 1-100))

Immunoprecipitation of over-expressed GFP-tagged protein in 293T cells incubated using GFP-tag antibody. (GFP 抗体 (AA 1-100))

3 images

(3 references)

产品编号 ABIN3020573

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
抗原表位
16

15

10

8

4

4

3

3

2

2

2

1

1

1

1

1

1
AA 1-100
适用
175

15

11

9
Aequorea victoria
宿主
63

62

35

15

9

5

1

1
小鼠
Compatible Secondaries
克隆类型
115

71
单克隆
标记
81

13

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
应用范围
149

76

45

33

25

25

25

21

14

13

13

12

12

5

4

4

2

2

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF)

序列

MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF

产品特性

Tag Antibodies

纯化方法

Affinity purification

免疫原

A synthetic peptide corresponding to a sequence within amino acids 1-100 to the N-terminus of GFP protein.

亚型

IgG

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应用备注

WB,1:2000 - 1:5000,IF,1:50 - 1:100

限制

仅限研究用
缓冲液

PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze / thaw cycles

储存条件

-20 °C

储存方法

Store at -20°C. Avoid freeze / thaw cycles.
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Yang, Yang, Sun, Chen, Xu, Lai, Li, Wang, Bhattarai, Xiao, Sun, Zhu, Ma, Adhikari, Sun, Hao, Zhang, Huang, Huang, Jiang, Zhao, Wang, Sun, Yang: "5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m5C reader." in: Cell research, Vol. 27, Issue 5, pp. 606-625, (2018) (PubMed).

Zhang, Lu, Liu, Cui, Wang: "Primate-specific miR-603 is implicated in the risk and pathogenesis of Alzheimer's disease." in: Aging, Vol. 8, Issue 2, pp. 272-90, (2016) (PubMed).
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

背景

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. It has been used in modified forms to make biosensors, and many animals have been created that express GFP, which demonstrates a proof of concept that a gene can be expressed throughout a given organism, in selected organs, or in cells of interest. GFP can be introduced into animals or other species through transgenic techniques, and maintained in their genome and that of their offspring. To date, GFP has been expressed in many species, including bacteria, yeasts, fungi, fish and mammals, including in human cells.,GFP,GFP tag,GFP-tag,GFP

分子量

27 kDa