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GFP 抗体 (AA 1-238)

Name: GFP 抗体 (AA 1-238)
Brand: antibodies-online
SKU: ABIN1512944
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, IF 宿主: 兔 Polyclonal unconjugated

Western blot analysis of extracts of normal 293T cells and 293T transfected with GFP-tagged fusion protein,using GFP antibody (ABIN3020570, ABIN3020571, ABIN3020572 and ABIN1512944) at 1:1000 dilution. (GFP 抗体 (AA 1-238))

Immunofluorescence analysis of 293T and 293T-GFP cells using Rabbit anti GFP-Tag pAb (ABIN3020570, ABIN3020571, ABIN3020572 and ABIN1512944) at dilution of 1:50 (40x lens). (GFP 抗体 (AA 1-238))

Western blot analysis of extracts of normal Eukaryotic expression of GFP and Eukaryotic expression of GFP transfected with GFP Protein, using GFP antibody (ABIN3020570, ABIN3020571, ABIN3020572 and ABIN1512944) at 1:20000 dilution. (GFP 抗体 (AA 1-238))

3 images

(5 references)

产品编号 ABIN1512944

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
抗原表位
16

15

10

8

4

4

3

3

2

2

2

1

1

1

1

1

1

1
AA 1-238
适用
176

15

11

9
Aequorea victoria
宿主
64

62

35

15

9

5

1

1
兔
Compatible Secondaries
克隆类型
115

72
多克隆
标记
82

13

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
应用范围
150

76

46

33

25

25

25

21

14

13

13

12

12

5

4

4

2

2

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF)

序列

MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK

产品特性

Tag Antibodies

纯化方法

Affinity purification

免疫原

Recombinant protein containing a sequence corresponding to amino acids 1-238 of GFP protein.

亚型

IgG

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应用备注

WB,1:2000 - 1:3000,IF,1:50 - 1:200

限制

仅限研究用
缓冲液

PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze / thaw cycles

储存条件

-20 °C

储存方法

Store at -20°C. Avoid freeze / thaw cycles.
Wang, Long, Chen, Fan, Wang, Li, Wu, Chen: "Inhibition of H3K4 demethylation induces autophagy in cancer cell lines." in: Biochimica et biophysica acta. Molecular cell research, Vol. 1864, Issue 12, pp. 2428-2437, (2018) (PubMed).

Li, Lu, Li, Yuan, Fu: "A ROP2-RIC1 pathway fine-tunes microtubule reorganization for salt tolerance in Arabidopsis." in: Plant, cell & environment, Vol. 40, Issue 7, pp. 1127-1142, (2017) (PubMed).

Zheng, Zhou, Zhang, Thu, Xie, Lu, Pang, Xue, Xu, Chen, Chen, Li, Xu: "Anhydroicaritin improves diet-induced obesity and hyperlipidemia and alleviates insulin resistance by suppressing SREBPs activation." in: Biochemical pharmacology, Vol. 122, pp. 42-61, (2017) (PubMed).

Zhao, Jing, Chen, Liu, Su, Liu, Gao, Yi, Wen, Ma, Tu, Zou, Fu, Shen: "Tribenuron-Methyl Induces Male Sterility through Anther-Specific Inhibition of Acetolactate Synthase Leading to Autophagic Cell Death." in: Molecular plant, Vol. 8, Issue 12, pp. 1710-24, (2016) (PubMed).

Zhu, Wang, Ju, Zhu, Yao, Wang, Wu, Li: "WDR82 Negatively Regulates Cellular Antiviral Response by Mediating TRAF3 Polyubiquitination in Multiple Cell Lines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 11, pp. 5358-66, (2016) (PubMed).
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

背景

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. It has been used in modified forms to make biosensors, and many animals have been created that express GFP, which demonstrates a proof of concept that a gene can be expressed throughout a given organism, in selected organs, or in cells of interest. GFP can be introduced into animals or other species through transgenic techniques, and maintained in their genome and that of their offspring. To date, GFP has been expressed in many species, including bacteria, yeasts, fungi, fish and mammals, including in human cells.,GFP,GFP tag,GFP-tag,GFP

分子量

27 kDa

UniProt

P42212