(15 references)-
- 抗原 See all His Tag 抗体
- His Tag
- 适用
- 请咨询
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This His Tag antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
- 序列
- HHHHHH
- 纯化方法
- Affinity purification
- 免疫原
- A 6×His synthetic peptide (KLH-coupled).
- 亚型
- IgG
- Top Product
- Discover our top product His Tag Primary Antibody
-
anti-His Tag antibody
WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA, IP, IHC, FACS 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibody适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated
anti-His Tag antibody (HRP)WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 HRP
anti-His Tag (C-Term) antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibody (Biotin)WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 Biotin
anti-His Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated
anti-His Tag antibody (DyLight 649)WB, FM, FLISA 宿主: 小鼠 Monoclonal 33D10-D2 DyLight 649
-
- 应用备注
- WB: 1:5000, IF: 1:2000, IP: 1:100
- 限制
- 仅限研究用
-
- 缓冲液
- PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid freeze / thaw cycles
- 储存条件
- -20 °C / -80 °C
-
-
: "InsP3R-SEC5 interaction on phagosomes modulates innate immunity to Candida albicans by promoting cytosolic Ca2+ elevation and TBK1 activity." in: BMC biology, Vol. 16, Issue 1, pp. 46, (2018) (PubMed).
: "The synchronous TAG production with the growth by the expression of chloroplast transit peptide-fused ScPDAT in Chlamydomonas reinhardtii." in: Biotechnology for biofuels, Vol. 11, pp. 156, (2018) (PubMed).
: "The calcium-dependent kinase OsCPK24 functions in cold stress responses in rice." in: Journal of integrative plant biology, Vol. 60, Issue 2, pp. 173-188, (2018) (PubMed).
: "Enhanced bacterial cellulose production by Gluconacetobacter xylinus via expression of Vitreoscilla hemoglobin and oxygen tension regulation." in: Applied microbiology and biotechnology, Vol. 102, Issue 3, pp. 1155-1165, (2018) (PubMed).
: "Effects of environmental factors on C-type lectin recognition to zooxanthellae in the stony coral Pocillopora damicornis." in: Fish & shellfish immunology, Vol. 79, pp. 228-233, (2018) (PubMed).
: "Identification of a clip domain serine proteinase involved in immune defense in Chinese mitten crab Eriocheir sinensis." in: Fish & shellfish immunology, Vol. 74, pp. 332-340, (2018) (PubMed).
: "Transgenic Eimeria magna Pérard, 1925 Displays Similar Parasitological Properties to the Wild-type Strain and Induces an Exogenous Protein-Specific Immune Response in Rabbits (Oryctolagus cuniculus ..." in: Frontiers in immunology, Vol. 8, pp. 2, (2017) (PubMed).
: "Molecular Characterization, Tissue Distribution and Expression, and Potential Antiviral Effects of TRIM32 in the Common Carp (Cyprinus carpio)." in: International journal of molecular sciences, Vol. 17, Issue 10, (2017) (PubMed).
: "Comparative study of two single CRD C-type lectins, CgCLec-4 and CgCLec-5, from pacific oyster Crassostrea gigas." in: Fish & shellfish immunology, Vol. 59, pp. 220-232, (2017) (PubMed).
: "Characterization and biological function analysis of the TRIM47 gene from common carp (Cyprinus carpio)." in: Gene, Vol. 627, pp. 188-193, (2017) (PubMed).
: "Construction of a series of pCS2+ backbone-based Gateway vectors for overexpressing various tagged proteins in vertebrates." in: Acta biochimica et biophysica Sinica, Vol. 48, Issue 12, pp. 1128-1134, (2017) (PubMed).
: "Ubiquitin-specific Protease 15 Negatively Regulates Virus-induced Type I Interferon Signaling via Catalytically-dependent and -independent Mechanisms." in: Scientific reports, Vol. 5, pp. 11220, (2016) (PubMed).
: "Heterologous expression and comparative characterization of vacuolar invertases from Cu-tolerant and non-tolerant populations of Elsholtzia haichowensis." in: Plant cell reports, Vol. 34, Issue 10, pp. 1781-90, (2016) (PubMed).
: "Pollen developmental defects in ZD-CMS rice line explored by cytological, molecular and proteomic approaches." in: Journal of proteomics, Vol. 108, pp. 110-23, (2015) (PubMed).
: "Glycoprotein E2 of classical swine fever virus expressed by baculovirus induces the protective immune responses in rabbits." in: Vaccine, Vol. 32, Issue 49, pp. 6607-13, (2015) (PubMed).
-
: "InsP3R-SEC5 interaction on phagosomes modulates innate immunity to Candida albicans by promoting cytosolic Ca2+ elevation and TBK1 activity." in: BMC biology, Vol. 16, Issue 1, pp. 46, (2018) (PubMed).
-
- 抗原
- His Tag
- 别名
- His-Tag (His Tag 产品)
- 物质类
- Tag
- 背景
- Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage, such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni++- chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site.
-
You are here:
