NLRP3 抗体 (AA 82-93)
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- 抗原 See all NLRP3 抗体
- NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
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抗原表位
- AA 82-93
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适用
- 人, 猴
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This NLRP3 antibody is un-conjugated
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应用范围
- ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human NLRP3. This antibody is expected to recognise all five reported isoforms (NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1). Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).
- 预测反应
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Bat, Panda, Pig (92%) Dog, Bovine, Rabbit (83%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide C-DLYEKAKRDEPK from an internal region of human NLRP3 (NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Bat, Panda, Pig (92%), Dog, Bovine, Rabbit (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NLRP3 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:64000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
- 别名
- NALP3 / NLRP3 (NLRP3 产品)
- 背景
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Name/Gene ID: NLRP3
Synonyms: NLRP3, AGTAVPRL, AII, C1orf7, Caterpiller protein 1.1, Cryopyrin, FCAS, FCU, PYPAF1, NALP3, CIAS1, CLR1.1, MWS - 基因ID
- 114548
- NCBI登录号
- NP_004886, NP_899632, NP_001230062, NP_001120933, NP_001120934
- UniProt
- Q96P20
- 途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome
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