PKC beta 抗体 (AA 607-671)
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- 抗原 See all PKC beta (PRKCB) 抗体
- PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))
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抗原表位
- AA 607-671
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适用
- 人, 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This PKC beta antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
- 特异性
- Recognizes human and rat PKCb1. Selected for its ability to recognize PKCb1 in the applications listed.
- 纯化方法
- Affinity purified
- 免疫原
- E. coli-derived rhPKCb1 (aa607-671).
- 亚型
- IgG
- Top Product
- Discover our top product PRKCB Primary Antibody
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- 应用备注
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Approved: ELISA, ICC, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect PKC 1. The detection limit for rhPKC 1 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect PKC 1 in neural progenitor cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human PKC 1. The detection limit for rhPKC 1 is approximately 0.3 ng/well. In this format, this antibody shows less than 1 % cross-reactivity with rhPKC. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- deionized water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized.
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- 抗原
- PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))
- 别名
- PRKCB / PKC-Beta (PRKCB 产品)
- 别名
- PKC-beta antibody, PKCB antibody, PRKCB1 antibody, PRKCB2 antibody, A130082F03Rik antibody, PKC-Beta antibody, Pkcb antibody, Prkcb1 antibody, Prkcb2 antibody, PKC-B antibody, Prkcb antibody, prkcb1 antibody, zgc:63591 antibody, PKC antibody, PRKCB_tv2 antibody, Pkc53E antibody, prkcb1l antibody, zgc:64063 antibody, protein kinase C beta antibody, protein kinase C, beta antibody, protein kinase C, beta b antibody, protein kinase C, beta a antibody, PRKCB antibody, Prkcb antibody, prkcbb antibody, prkcba antibody
- 背景
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Name/Gene ID: PRKCB
Subfamily: PKC
Family: Protein Kinase
Synonyms: PRKCB, PKC beta 2, Pkc betaII, PRKCB2, Protein kinase c beta, Protein kinase c beta 2, Protein kinase c beta i, Protein kinase C beta type, Protein kinase C, beta, Protein kinase C, beta 1, PRKCB1, Pkc betaI, PKC-beta, PKCB, Pkcbeta, PkcbetaI, PKC-B, PkcbetaII, Protein kinase c beta 1, Protein kinase c beta II - 基因ID
- 5579
- UniProt
- P05771
- 途径
- WNT signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling
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