LPIN3 抗体 (AA 428-529)
LPIN3
适用: 小鼠
WB, IHC, ELISA, ICC
宿主: 山羊
Polyclonal
unconjugated
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- 抗原 See all LPIN3 抗体
- LPIN3 (Lipin 3 (LPIN3))
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抗原表位
- AA 428-529
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This LPIN3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)
- 特异性
- Selected for its ability to recognize Lipin 3 in the applications listed. In direct ELISAs and Western Blots, this antibody shows ~50 % cross-reactivity with rhLipin 3 and less than 5 % cross-reactivity with rhLipin 1 and rhLipin 2.
- 纯化方法
- Affinity purified
- 免疫原
- E. coli-derived recombinant mouse Lipin 3 (aa428-529).
- 亚型
- IgG
- Top Product
- Discover our top product LPIN3 Primary Antibody
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anti-Lipin 3 (LPIN3) (Internal Region) antibody
Verified LPIN3 适用: 人 IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse rmLipin 3. The detection limit for rmLipin 3 is approximately 2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect Lipin 3 on CCD-18co normal human colon fibroblasts. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4?C followed by donkey anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Immunohistochemistry: 10 μg/mL to detect Lipin 3 in cryosections of adult mouse intestine. Tissue was fixed overnight in 4 % PFA + 14 % picric acid in PBS at 4?C. Sections were blocked with 10 % donkey serum and 0.1 % Triton in 1 % BSA in PBS for 45 minutes at RT. After blocking, sections were incubated with primary antibody for 3 hours at RT followed by donkey anti-goat lgG at RT for 1 hour. Between each step, sections were washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse rmLipin 3. The detection limit for rmLipin 3 is approximately 0.05 ng/well. - 说明
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Target Species of Antibody: Mouse
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Sterile PBS.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, 5 % trehalose
- 储存条件
- -20 °C
- 储存方法
- Lyophilized powder may be stored at -20°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
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- 抗原
- LPIN3 (Lipin 3 (LPIN3))
- 别名
- LPIN3 / LIPIN-3 (LPIN3 产品)
- 别名
- MGC82840 antibody, LPIN3 antibody, DKFZp469D075 antibody, LIPN3L antibody, SMP2 antibody, dJ450M14.2 antibody, dJ450M14.3 antibody, dJ620E11.2 antibody, 9130206L11Rik antibody, AA438110 antibody, AV236139 antibody, mKIAA4023 antibody, lipin 3 L homeolog antibody, lipin 3 antibody, lpin3.L antibody, LPIN3 antibody, LOAG_08747 antibody, lpin3 antibody, Lpin3 antibody
- 背景
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Name/Gene ID: LPIN3
Synonyms: LPIN3, DJ620E11.2, DJ450M14.2, Lipin-3, Lipin 3, Lipin 3-like, Lipin-3-like, SMP2, DJ450M14.3, LIPN3L - 基因ID
- 64900
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