RFP 抗体
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- 抗原 See all RFP 抗体
- RFP (Red Fluorescent Protein (RFP))
- 适用
- Discosoma
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RFP antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 原理
- Anti-RFP / Red Fluorescent Protein Antibody
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Type of Immunogen: Fusion protein - Featured
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- 应用备注
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Approved: ELISA (1:700000), IHC, WB (1:2000 - 1:10000)
Usage: Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid freeze-thaw cycles.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C.
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- 抗原
- RFP (Red Fluorescent Protein (RFP))
- 别名
- RFP (RFP 产品)
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