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FITC 抗体 (HRP)
FITC
ELISA, WB, IHC
宿主: 山羊
Polyclonal
HRP
FITC抗体详情 (HRP)
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抗原
See all FITC products
FITC
(Fluorescein Isothiocyanate (FITC))
适用
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宿主
All hosts for FITC 抗体
山羊
克隆类型
All clonalities for FITC 抗体
多克隆
标记
All conjugates for FITC 抗体
This FITC antibody is conjugated to HRP
应用范围
All applications for FITC 抗体
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
纯化方法
Affinity chromatography purified
免疫原
FITC antibody (HRP) was raised in goat using fluorescein conjugated to goat IgG as the immunogen.
Alternatives
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使用细节
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应用备注
ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,000, WB: 1:2,000 - 1:5,000 Optimal conditions should be determined by the investigator.
限制
仅限研究用
贮存及处理
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状态
Lyophilized
浓度
Lot specific
缓冲液
Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % gentamycin sulfate. DO NOT add NaN3. Immunoglobulin and protease free.
储存液
Without preservative
注意事项
Avoid repeated freeze/thaw cycles. Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. Dilute only prior to immediate use.
储存条件
4 °C/-20 °C
储存方法
Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
FITC目标详情
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抗原
FITC
(Fluorescein Isothiocyanate (FITC))
别名
FITC (FITC 产品 )
物质类
Chemical
背景
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.
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