This antibody is reactive with Hsp65 (GroEL) of M. tuberculosis. Does not react with M. bovis, M. avium, M. phlei, M. parafortuitum, Rhodococcus sp., B. subtilis, S. pneumoniae, and E. coli.
产品特性
Synonyms: 60 kDa chaperonin 2, Protein Cpn60-2, groEL protein 2, Cell wall protein A, Antigen A,groL2, groEL-2, groEL2, hsp65, Rv0440, MT0456, MTV037.04
纯化方法
Protein A chromatography
纯度
> 90 % pure
免疫原
Purified protein derivative (PPD)
克隆位点
BDI578
亚型
IgG2a
应用备注
ELISA. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0,1 mg/mL (OD280nm, E0.1% = 1.4)
缓冲液
0.01 M PBS, pH 7.2 containing 0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Store the antibody at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
有效期
12 months
抗原
Heat Shock Protein 65 (HSP65)
别名
Heat Shock Protein 65 / HSP65
别名
CPN60 antibody, GROEL antibody, HLD4 antibody, HSP-60 antibody, HSP60 antibody, HSP65 antibody, HuCHA60 antibody, SPG13 antibody, heat shock protein family D (Hsp60) member 1 antibody, HSPD1 antibody
背景
Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure, most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.Synonyms: 60 kDa chaperonin 2, Antigen A, Cell wall protein A, MT0456, MTV037.04, Protein Cpn60-2, Rv0440, groEL protein 2, groEL-2, groEL2, groL2, hsp65