GAPDH 抗体
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- 抗原 See all GAPDH 抗体
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GAPDH antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 交叉反应
- Bacteria, C. elegans, Chanos chanos, 小鸡, Cow, 犬, Drosophila melanogaster, E. coli, 鱼, Hamster, 人, Insect, 猴, Mosquito, 小鼠, Nematode, Ochotona princeps, Pig, 植物, 兔, 大鼠, Saccharomyces cerevisiae, 斑马鱼
- 产品特性
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Rabbit Polyclonal antibody to GAPDH (glyceraldehyde-3-phosphate dehydrogenase)
GAPDH antibody - 纯化方法
- Purified by antigen-affinity chromatography.
- 免疫原
- Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.
- 亚型
- IgG
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- 应用备注
- WB: 1:5000-1:100000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- 说明
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Positive Control: PC-12 , Rat2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , E.coli M15
Validation: Orthogonal
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.11 mg/mL
- 缓冲液
- 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7), 0.025 % ProClin 300
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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MiR-874-3p represses the migration and invasion yet promotes the apoptosis and cisplatin sensitivity via being sponged by long intergenic non-coding RNA 00922 (LINC00922) and targeting ..." in: Bioengineered, Vol. 13, Issue 3, pp. 7082-7104, (2022) (PubMed).
: "Vitamin D protects against immobilization-induced muscle atrophy via neural crest-derived cells in mice." in: Scientific reports, Vol. 10, Issue 1, pp. 12242, (2020) (PubMed).
: "Effects of miR‑195‑5p on cell proliferation and apoptosis in gestational diabetes mellitus via targeting EZH2." in: Molecular medicine reports, Vol. 22, Issue 2, pp. 803-809, (2020) (PubMed).
: "Mutated SASH1 promotes Mitf expression in a heterozygous mutated SASH1 knock‑in mouse model." in: International journal of molecular medicine, Vol. 46, Issue 3, pp. 1118-1134, (2020) (PubMed).
: "The Antihistamine Deptropine Induces Hepatoma Cell Death through Blocking Autophagosome-Lysosome Fusion." in: Cancers, Vol. 12, Issue 6, (2020) (PubMed).
: "Long non-coding RNA NPBWR1-2 affects the development of ovarian cancer via multiple microRNAs." in: Oncology letters, Vol. 20, Issue 1, pp. 685-692, (2020) (PubMed).
: "Berberine Derivatives Suppress Cellular Proliferation and Tumorigenesis In Vitro in Human Non-Small-Cell Lung Cancer Cells." in: International journal of molecular sciences, Vol. 21, Issue 12, (2020) (PubMed).
: "Reciprocal Regulation Between Indoleamine 2,3-Dioxigenase 1 and Notch1 Involved in Radiation Response of Cervical Cancer Stem Cells." in: Cancers, Vol. 12, Issue 6, (2020) (PubMed).
: "Cisplatin Protein Binding Partners and Their Relevance for Platinum Drug Sensitivity." in: Cells, Vol. 9, Issue 6, (2020) (PubMed).
: "HIV-1 Tat - TLR4/MD2 interaction drives the expression of IDO-1 in monocytes derived dendritic cells through NF-κB dependent pathway." in: Scientific reports, Vol. 10, Issue 1, pp. 8177, (2020) (PubMed).
: "Exposure to Zinc Oxide Nanoparticles Disrupts Endothelial Tight and Adherens Junctions and Induces Pulmonary Inflammatory Cell Infiltration." in: International journal of molecular sciences, Vol. 21, Issue 10, (2020) (PubMed).
: "Proimmunogenic impact of MEK inhibition synergizes with agonist anti-CD40 immunostimulatory antibodies in tumor therapy." in: Nature communications, Vol. 11, Issue 1, pp. 2176, (2020) (PubMed).
: "Sequential Interferon β-Cisplatin Treatment Enhances the Surface Exposure of Calreticulin in Cancer Cells via an Interferon Regulatory Factor 1-Dependent Manner." in: Biomolecules, Vol. 10, Issue 4, (2020) (PubMed).
: "A Curcumin Derivative Activates TFEB and Protects Against Parkinsonian Neurotoxicity inVitro." in: International journal of molecular sciences, Vol. 21, Issue 4, (2020) (PubMed).
: "A Diterpenoid, 14-Deoxy-11, 12-Didehydroandrographolide, in Andrographis paniculata Reduces Steatohepatitis and Liver Injury in Mice Fed a High-Fat and High-Cholesterol Diet." in: Nutrients, Vol. 12, Issue 2, (2020) (PubMed).
: "Effects of Andrographolide on Intracellular pH Regulation, Cellular Migration, and Apoptosis in Human Cervical Cancer Cells †." in: Cancers, Vol. 12, Issue 2, (2020) (PubMed).
: "A stress response p38 MAP kinase inhibitor SB202190 promoted TFEB/TFE3-dependent autophagy and lysosomal biogenesis independent of p38." in: Redox biology, Vol. 32, pp. 101445, (2020) (PubMed).
: "Dopamine D3 receptor and GSK3β signaling mediate deficits in novel object recognition memory within dopamine transporter knockdown mice." in: Journal of biomedical science, Vol. 27, Issue 1, pp. 16, (2020) (PubMed).
: "Progerin in muscle leads to thermogenic and metabolic defects via impaired calcium homeostasis." in: Aging cell, Vol. 19, Issue 2, pp. e13090, (2020) (PubMed).
: "Lovastatin-mediated MCF-7 cancer cell death involves LKB1-AMPK-p38MAPK-p53-survivin signalling cascade." in: Journal of cellular and molecular medicine, Vol. 24, Issue 2, pp. 1822-1836, (2020) (PubMed).
: "
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MiR-874-3p represses the migration and invasion yet promotes the apoptosis and cisplatin sensitivity via being sponged by long intergenic non-coding RNA 00922 (LINC00922) and targeting ..." in: Bioengineered, Vol. 13, Issue 3, pp. 7082-7104, (2022) (PubMed).
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- 抗原
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- 别名
- glyceraldehyde-3-phosphate dehydrogenase (GAPDH 产品)
- 别名
- G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody
- 背景
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The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
Cellular Localization: Cytoplasm , perinuclear region , Membrane - 分子量
- 36 kDa
- 基因ID
- 2597
- UniProt
- P04406
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