S1PR1 抗体 (C-Term)
(1 validation)-
- 抗原 See all S1PR1 抗体
- S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This S1PR1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 交叉反应
- 人, 小鼠
- 产品特性
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Rabbit Polyclonal antibody to EDG1 (sphingosine-1-phosphate receptor 1)
EDG1 antibody [C2C3], C-term - 纯化方法
- Purified by antigen-affinity chromatography.
- 免疫原
- Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EDG1. The exact sequence is proprietary.
- 亚型
- IgG
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anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (AA 51-150) antibody
S1PR1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (AA 311-382) antibodyS1PR1 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (AA 1-47) antibodyS1PR1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E12 unconjugated
anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) antibodyS1PR1 适用: 人 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 1E3H5 unconjugated
anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (AA 1-47) antibodyS1PR1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F11 unconjugated
anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (Internal Region) antibodyS1PR1 适用: 人, 大鼠 WB, ELISA, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (Ser311) antibodyS1PR1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (AA 1-382) antibodyS1PR1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (AA 17-30), (Extracellular), (N-Term) antibodyS1PR1 适用: 人, 小鼠, 大鼠 WB, IF, IHC, ICC, LCI 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (pThr236) antibodyS1PR1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- 说明
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Positive Control: HepG2
- 限制
- 仅限研究用
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- by
- Memorial Sloan-Kettering Cancer Center, Parada lab
- No.
- #100014
- 日期
- 2016.05.11
- 抗原
- Sphingosine-1-Phosphate Receptor 1 (S1PR1)
- Lot Number
- 39471
- Method validated
- Immunofluorescence
- Positive Control
- Wild-type mouse adult brain cryo-sections
- Negative Control
- Rabbit IgG
- Notes
- The antibody showed signals along the subventricular zone, which is consistent with the RNA expression analysis.
- Primary Antibody
- ABIN2857018
- Secondary Antibody
- Donkey-anti-Rabbit-Cy3
- Full Protocol
- Wild-type mouse adult brain was dissected, fixed in 4% PFA for over/night, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
- Immunostaining for Cryo Sections:
- Dry slides at 50-55°C for 15-30min (or RT for 30min).
- Wash with PBS 5min X 3 (shake gently).
- Block with 10% Normal Donkey Serum (NDS) at RT for 1h.
- Incubate with ABIN2857018 diluted 1:10 - 1:800 in 2% NDS at 4°C overnight.
- Wash with PBS 5min X 3.
- Incubate with the secondary antibody Donkey-anti-Rabbit-Cy3 together with DAPI 1:100 at RT for 1h (prepared in 2% NDS), cover the chamber with foil.
- Wash with PBS 5min X 3.
- Add three drops of water-based Immu-mount.
- Cover with 24 X 60 cover glass.
- Imaging:
- Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.
- Experimental Notes
- ABIN2857018 worked the best at 1:200. At 1:400, or 1:800, it seems to lose some signals in some cells.
生效 #100014 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![IF validation of S1PR1 antibody ABIN2857018 on mouse brain sections.
A. ABIN2857018 diluted 1:200 (red), DAPI counterstaining (blue).
B. Dilution factor 1:100. C. Dilution factor 1:200. D. Dilution factor 1:400.]()
IF validation of S1PR1 antibody ABIN2857018 on mouse brain sections.
A. ABIN2857018 diluted 1:200 (red), DAPI counterstaining (blue).
B. Dilution factor 1:100. C. Dilution factor 1:200. D. Dilution factor 1:400.
Full Methods -
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- 抗原
- S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))
- 别名
- sphingosine-1-phosphate receptor 1 (S1PR1 产品)
- 别名
- CD363 antibody, CHEDG1 antibody, D1S3362 antibody, ECGF1 antibody, EDG-1 antibody, EDG1 antibody, S1P1 antibody, AI849002 antibody, Edg1 antibody, Lpb1 antibody, S1p antibody, S1p1 antibody, S1P receptor 1 antibody, S1P receptor Edg-1 antibody, edg1 antibody, etID43307.22 antibody, s1p1 antibody, wu:fb98f05 antibody, s1pr1 antibody, xts1p1 antibody, sphingosine-1-phosphate receptor 1 antibody, sphingosine-1-phosphate receptor 1 L homeolog antibody, S1PR1 antibody, S1pr1 antibody, s1pr1 antibody, s1pr1.L antibody
- 背景
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The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion.
Cellular Localization: Cell membrane, Multi-pass membrane protein - 分子量
- 43 kDa
- 基因ID
- 1901
- UniProt
- P21453
- 途径
- Signaling Events mediated by VEGFR1 and VEGFR2
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