Pyruvate Kinase 抗体 (Center)
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- 抗原 See all Pyruvate Kinase (PK) 抗体
- Pyruvate Kinase (PK)
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抗原表位
- Center
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适用
- 人, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Pyruvate Kinase antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 特异性
- This antibody is raised against the R-Type isoform. Based on a BLAST result, it may also react with the L-Type isoform.
- 产品特性
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Rabbit Polyclonal antibody to Pyruvate Kinase (liver/RBC) (pyruvate kinase, liver and RBC)
Pyruvate Kinase (liver/RBC) antibody - 纯化方法
- Purified by antigen-affinity chromatography.
- 免疫原
- Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary.
- 亚型
- IgG
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anti-Pyruvate Kinase (PK) (AA 476-505), (C-Term) antibody
PK 适用: 人, 小鼠, 大鼠, 猴 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase (PK) antibodyPK 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase (PK) antibody (Biotin)PK 适用: 兔 WB, ELISA 宿主: 山羊 Polyclonal Biotin
anti-Pyruvate Kinase (PK) (AA 121-151), (N-Term) antibodyPK 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase (PK) (AA 476-505), (C-Term) antibodyPK 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:1000-1:10000*
- 说明
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Positive Control: 293T , A431 , HeLa , HepG2 , A375 , rat liver
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- 抗原
- Pyruvate Kinase (PK)
- 别名
- Pyruvate Kinase (PK 产品)
- 别名
- CG7070 antibody, Dmel\\CG7070 antibody, PYK antibody, Pyk antibody, PYK3b antibody, PYK3c antibody, Pyruvate kinase antibody, PK antibody, pyruvate kinase antibody, pyruvate kinase (PK) antibody, Pyruvate kinase (PK) antibody, pyruvate kinase PKM antibody, testis expressed 45 antibody, PyK antibody, pykA antibody, PYK3a|PYK3b|PYK3c antibody, pyk antibody, pyk1 antibody, pk antibody, LOC100534543 antibody, TEX45 antibody
- 背景
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The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.
- 分子量
- 62 kDa
- 基因ID
- 5313
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