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PKC alpha 抗体 (N-Term)

Name: PKC alpha 抗体 (N-Term)
Brand: antibodies-online
SKU: ABIN2856799
Availability: OutOfStock

PKCa 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

WB Image Whole cell extract (30 μg) was separated by 7. (PKC alpha 抗体 (N-Term))

WB Image Sample (30 ug of whole cell lysate) A: H1299 7. (PKC alpha 抗体 (N-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PKC alpha, antibody at 1:500 dilution. (PKC alpha 抗体 (N-Term))

IHC

3 images

产品编号 ABIN2856799

发货至: 中国

Datasheet as PDF

抗原 See all PKC alpha (PKCa) 抗体

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
抗原表位
23

21

16

12

9

9

8

7

7

6

5

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
161

89

66

5

5

3

3

2

2

1

1

1

1
人
宿主
159

10

1
兔
Compatible Secondaries
克隆类型
140

29
多克隆
标记
81

14

10

6

6

6

6

6

6

6

4

4

2

2

1

1

1

1

1

1

1

1

1

1

1
This PKC alpha antibody is un-conjugated
应用范围
113

66

53

22

18

16

15

15

14

7

5

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

交叉反应

人, 小鼠, 大鼠

产品特性

Rabbit Polyclonal antibody to PKC alpha (protein kinase C, alpha)
PKC alpha antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human PKC alpha. The exact sequence is proprietary.

亚型

IgG

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anti-Protein Kinase C, alpha (PKCa) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, IHC, IP, FACS, IF, ICC 宿主: 兔 Monoclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (AA 299-329) antibody
PKCa 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (pThr638) antibody
PKCa 适用: 人, 小鼠 WB, FACS, IHC (p), IF (cc) 宿主: 兔 Monoclonal 4B3 unconjugated

anti-Protein Kinase C, alpha (PKCa) (pThr638) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) antibody
PKCa 适用: 人, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (AA 299-329), (C-Term) antibody
PKCa 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB01012 unconjugated

anti-Protein Kinase C, alpha (PKCa) (C-Term) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) antibody
PKCa 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (AA 75-103), (N-Term) antibody
PKCa 适用: 人, 小鼠 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB20769 unconjugated

应用备注

WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: H1299 , Jurkat , U87-MG , SK-N-SH , IMR32 , SK-N-AS , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

限制

仅限研究用
状态

Liquid

浓度

0.69 mg/mL

缓冲液

0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
抗原

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

别名

protein kinase C alpha (PKCa 产品)

别名

pkc-alpha antibody, pkcalpha antibody, im:7139045 antibody, si:dkey-8l19.1 antibody, LOC100219195 antibody, AAG6 antibody, PKC-alpha antibody, PKCA antibody, PRKACA antibody, AI875142 antibody, Pkca antibody, PKRCA antibody, protein kinase C alpha antibody, protein kinase C, alpha L homeolog antibody, protein kinase C, alpha antibody, PRKCA antibody, prkca.L antibody, prkca antibody, Prkca antibody

背景

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

分子量

77 kDa

基因ID

5578

UniProt

P17252

途径

WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling