RAG2 抗体 (Center)
-
- 抗原 See all RAG2 抗体
- RAG2 (Recombination Activating Gene 2 (RAG2))
-
抗原表位
- Center
-
适用
- 人, 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This RAG2 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 交叉反应
- 小鼠, 猪, 兔, 大鼠, 牛
- 交叉反应 （详细）
- Mouse (85 %), Pig (90 %), Rabbit (91 %), Rat (86 %), Bovine (91 %)
- 产品特性
-
Rabbit Polyclonal antibody to RAG2 (recombination activating gene 2)
RAG2 antibody - 纯化方法
- Purified by antigen-affinity chromatography.
- 免疫原
- Recombinant protein encompassing a sequence within the center region of human RAG2. The exact sequence is proprietary.
- 亚型
- IgG
-
anti-Recombination Activating Gene 2 (RAG2) (AA 1-527) antibody
RAG2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 428-527) antibodyRAG2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G8 unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 258-527) antibodyRAG2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 498-527), (C-Term) antibodyRAG2 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 1-527) antibodyRAG2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (N-Term) antibodyRAG2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 350-527) antibodyRAG2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4D5 unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 296-415) antibodyRAG2 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) antibodyRAG2 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (C-Term) antibodyRAG2 适用: 人, Cow, 犬, Pig WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
- 说明
-
Positive Control: Molt-4 , rat thymus
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.37 mg/mL
- 缓冲液
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
-
- 抗原
- RAG2 (Recombination Activating Gene 2 (RAG2))
- 别名
- RAG2 (RAG2 产品)
- 别名
- RAG-2 antibody, Rag-2 antibody, rag-2 antibody, zgc:136743 antibody, recombination activating 2 antibody, recombination activating gene 2 antibody, recombination activating gene 2 S homeolog antibody, RAG2 antibody, Rag2 antibody, rag2 antibody, rag2.S antibody
- 背景
-
This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.
Cellular Localization: Nucleus - 分子量
- 59 kDa
- 基因ID
- 5897
- 途径
- Chromatin Binding, Production of Molecular Mediator of Immune Response
-
