CRYbA4 抗体
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- 抗原 See all CRYbA4 抗体
- CRYbA4 (Crystallin, beta A4 (CRYbA4))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CRYbA4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 交叉反应
- 人, 小鼠
- 产品特性
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Rabbit Polyclonal antibody to Beta crystallin A4 (crystallin, beta A4)
beta A4 Crystallin antibody [N1C3-2] - 纯化方法
- Purified by antigen-affinity chromatography.
- 免疫原
- Recombinant protein encompassing a sequence within the center region of human beta A4 Crystallin. The exact sequence is proprietary.
- 亚型
- IgG
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- 应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- 说明
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Positive Control: Mouse brain , 293T , A431 , HeLa , HepG2 , H1299
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- 抗原
- CRYbA4 (Crystallin, beta A4 (CRYbA4))
- 别名
- crystallin beta A4 (CRYbA4 产品)
- 背景
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Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families, beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, is part of a gene cluster with beta-B1, beta-B2, and beta-B3.
- 分子量
- 22 kDa
- 基因ID
- 1413
- UniProt
- P53673
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