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RPA1 抗体 (C-Term)

Name: RPA1 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN2856633
Availability: OutOfStock

RPA1 适用: 人 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

WB Image Sample (50 ug of whole cell lysate) A: mouse liver 7. (RPA1 抗体 (C-Term))

IP Image Immunoprecipitation of RPA70 protein from 293T whole cell extracts using 5 μg of RPA70 antibody [C1C3], Western blot analysis was performed using RPA70 antibody [C1C3], EasyBlot anti-Rabbit IgG was used as a secondary reagent. (RPA1 抗体 (C-Term))

IHC-P Image RPA70 antibody [C1C3] detects RPA1 protein at nucleus in mouse brain by immunohistochemical analysis. (RPA1 抗体 (C-Term))

IHC

IHC-P Image RPA70 antibody [C1C3] detects RPA70 protein at cytoplasm and nucleus in human lung papillary adenocarcinoma by immunohistochemical analysis. (RPA1 抗体 (C-Term))

IHC

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA 70 kDa subunit , antibody at 1:100 dilution. (RPA1 抗体 (C-Term))

IHC

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit , antibody at 1:200 dilution. (RPA1 抗体 (C-Term))

WB Image RPA70 antibody detects RPA70 protein by western blot analysis. (RPA1 抗体 (C-Term))

7 images

产品编号 ABIN2856633

发货至: 中国

Datasheet as PDF

抗原 See all RPA1 抗体

RPA1 (Replication Protein A1, 70kDa (RPA1))
抗原表位
16

9

9

6

6

3

3

2

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
78

24

14

4

3

3

2

2

2

2

2

2

2

2

1
人
宿主
65

12

1

1
兔
Compatible Secondaries
克隆类型
66

13
多克隆
标记
43

5

5

4

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This RPA1 antibody is un-conjugated
应用范围
66

32

20

14

13

12

12

11

9

7

3

2

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

交叉反应

人, 小鼠

产品特性

Rabbit Polyclonal antibody to RPA70 (replication protein A1, 70 kDa)
RPA70 antibody [C1C3]

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human RPA70. The exact sequence is proprietary.

亚型

IgG

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anti-Replication Protein A1, 70kDa (RPA1) antibody
RPA1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 小鼠 Monoclonal 4C4 unconjugated

anti-Replication Protein A1, 70kDa (RPA1) (AA 1-616) antibody
RPA1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Replication Protein A1, 70kDa (RPA1) antibody
RPA1 适用: 人, 猴 WB, IF, FACS, IHC (p), EIA 宿主: 小鼠 Monoclonal 4C4 unconjugated

anti-Replication Protein A1, 70kDa (RPA1) antibody
RPA1 适用: 人, 小鼠 WB, IP, ICC 宿主: 小鼠 Monoclonal unconjugated

anti-Replication Protein A1, 70kDa (RPA1) (AA 1-616) antibody
RPA1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Replication Protein A1, 70kDa (RPA1) (Internal Region) antibody
RPA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Replication Protein A1, 70kDa (RPA1) (AA 1-240) antibody
RPA1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Replication Protein A1, 70kDa (RPA1) (AA 367-616) antibody
RPA1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Replication Protein A1, 70kDa (RPA1) (AA 2-616) antibody
RPA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: Mouse liver , 293T , A431 , HeLa , HepG2

限制

仅限研究用
状态

Liquid

浓度

0.4 mg/mL

缓冲液

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
抗原

RPA1 (Replication Protein A1, 70kDa (RPA1))

别名

replication protein A1 (RPA1 产品)

别名

HSSB antibody, MST075 antibody, REPA1 antibody, RF-A antibody, RP-A antibody, RPA70 antibody, Cb1-727 antibody, wu:fi14b08 antibody, zgc:55337 antibody, zgc:77092 antibody, RPA1 antibody, hssb antibody, mst075 antibody, repa1 antibody, rf-a antibody, rp-a antibody, rpa antibody, rpa70 antibody, LOC100230990 antibody, 5031405K23Rik antibody, 70kDa antibody, AA589576 antibody, AW557552 antibody, Rpa antibody, CG9633 antibody, D-RPA70 antibody, D-SSB antibody, DRP-A antibody, DmRPA antibody, Dmel\\CG9633 antibody, RPA antibody, RPA 70 antibody, RPA 70 kDa antibody, RpA70 antibody, Rpa-70 antibody, Ssb-70 antibody, dRP-A antibody, dmrpa1 antibody, i164 antibody, replication protein A1 antibody, replication protein A1, 70kDa antibody, replication protein A1 L homeolog antibody, Replication Protein A 70 antibody, RPA1 antibody, Rpa1 antibody, rpa1 antibody, rpa1.L antibody, RpA-70 antibody

背景

Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair, it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Cellular Localization: Nucleus

分子量

68 kDa

基因ID

6117

UniProt

P27694

途径

Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA