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Nemo-Like Kinase 抗体

NLK 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN2856544
发货至: 中国
  • 抗原 See all Nemo-Like Kinase (NLK) 抗体
    Nemo-Like Kinase (NLK)
    适用
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    宿主
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    克隆类型
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    多克隆
    标记
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    This Nemo-Like Kinase antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    交叉反应
    人, 小鼠
    产品特性
    Rabbit Polyclonal antibody to NLK (nemo-like kinase)
    NLK antibody [N3C3]
    纯化方法
    Purified by antigen-affinity chromatography.
    免疫原
    Recombinant protein encompassing a sequence within the center region of human NLK. The exact sequence is proprietary.
    亚型
    IgG
    Top Product
    Discover our top product NLK Primary Antibody
  • 应用备注
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
    说明

    Positive Control: Mouse brain , HeLa , NLK-transfected 293T

    Validation: Overexpression

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal
    储存液
    Thimerosal (Merthiolate)
    注意事项
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原
    Nemo-Like Kinase (NLK)
    别名
    nemo like kinase (NLK 产品)
    背景
    Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.

    Cellular Localization: Nucleus (By similarity) , Cytoplasm (By similarity)
    分子量
    58 kDa
    基因ID
    51701
    UniProt
    Q9UBE8
    途径
    Ubiquitin Proteasome Pathway
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