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RAD21 抗体 (Center)

Name: RAD21 抗体 (Center)
Brand: antibodies-online
SKU: ABIN2856242
Availability: OutOfStock

RAD21 适用: 人, 小鼠 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. (RAD21 抗体 (Center))

IHC-P Image Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using RAD21, antibody at 1:750 dilution. (RAD21 抗体 (Center))

IHC

IP Image Immunoprecipitation of RAD21 protein from Jurkat whole cell extracts using 5 μg of RAD21 antibody, Western blot analysis was performed using RAD21 antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (RAD21 抗体 (Center))

ICC/IF Image RAD21 antibody detects RAD21 protein at nucleus by immunofluorescent analysis. (RAD21 抗体 (Center))

IHC-P Image Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RAD21, antibody at 1:750 dilution. (RAD21 抗体 (Center))

IHC

IHC-P Image RAD21 antibody detects RAD21 protein at nucleus in human lung papillary adenocarcinoma by immunohistochemical analysis. (RAD21 抗体 (Center))

IHC

WB Image RAD21 antibody detects RAD21 protein by western blot analysis. (RAD21 抗体 (Center))

7 images

(1 reference)

产品编号 ABIN2856242

发货至: 中国

Datasheet as PDF

抗原 See all RAD21 抗体

RAD21 (RAD21 Homolog (RAD21))
抗原表位
16

12

5

3

2

1

1

1

1

1

1

1
Center
适用
54

31

30

6

5

5

5

4

4

3

2

2

2

2

1
人, 小鼠
宿主
47

5

1
兔
Compatible Secondaries
克隆类型
45

9
多克隆
标记
32

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This RAD21 antibody is un-conjugated
应用范围
46

16

13

13

10

9

9

6

5

4

3

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

产品特性

Rabbit Polyclonal antibody to RAD21 (RAD21 homolog (S. pombe))
RAD21 antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human RAD21. The exact sequence is proprietary.

亚型

IgG
anti-RAD21 Homolog (RAD21) antibody
RAD21 适用: 人 WB, IHC, IF, IP, ChIP 宿主: 兔 Polyclonal unconjugated

anti-RAD21 Homolog (RAD21) (AA 287-403) antibody
RAD21 适用: 人, 大鼠, 猴 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 1B6D1 unconjugated

anti-RAD21 Homolog (RAD21) (C-Term) antibody
RAD21 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-RAD21 Homolog (RAD21) (AA 373-422) antibody
RAD21 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 猴, 豚鼠, 小鸡, Bat, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated

anti-RAD21 Homolog (RAD21) antibody
RAD21 适用: 人 WB, ChIP 宿主: 兔 Monoclonal unconjugated

anti-RAD21 Homolog (RAD21) (C-Term) antibody
RAD21 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated

anti-RAD21 Homolog (RAD21) (C-Term) antibody
RAD21 适用: 人, 小鼠, Hamster WB, IF 宿主: 兔 Polyclonal unconjugated

anti-RAD21 Homolog (RAD21) (AA 531-631) antibody
RAD21 适用: 人, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-RAD21 Homolog (RAD21) (AA 501-535) antibody
RAD21 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal RB56184 unconjugated

anti-RAD21 Homolog (RAD21) (AA 178-227) antibody
RAD21 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 猴, 豚鼠, 小鸡, Bat, Pig WB 宿主: 兔 Polyclonal unconjugated

应用备注

Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

说明

Positive Control: K562 , THP-1 , HL-60 , NIH-3T3 , JC , BCL-1

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Ochiai, Hayashi, Umeda, Yoshimura, Harada, Shimizu, Nakano, Saitoh, Liu, Yamamoto, Okamura, Ohkawa, Kimura, Nikaido: "Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells." in: Science advances, Vol. 6, Issue 25, pp. eaaz6699, (2020) (PubMed).
抗原

RAD21 (RAD21 Homolog (RAD21))

别名

RAD21 (RAD21 产品)

别名

rad21 antibody, wu:fb55d02 antibody, wu:fb93f04 antibody, CDLS4 antibody, HR21 antibody, HRAD21 antibody, MCD1 antibody, NXP1 antibody, SCC1 antibody, hHR21 antibody, mKIAA0078 antibody, hr21 antibody, mcd1 antibody, nxp-1 antibody, nxp1 antibody, scc1 antibody, xrad21 antibody, RAD21 cohesin complex component antibody, RAD21 cohesin complex component a antibody, RAD21 cohesin complex component L homeolog antibody, RAD21 cohesin complex component S homeolog antibody, R-spondin 2 antibody, RAD21 antibody, rad21a antibody, rad21.L antibody, Rad21 antibody, rad21.S antibody, RSPO2 antibody

背景

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

Cellular Localization: Nucleus

分子量

72 kDa

基因ID

5885

途径

Positive Regulation of Endopeptidase Activity