PFKL 抗体 (Center)
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- 抗原 See all PFKL 抗体
- PFKL (Phosphofructokinase, Liver (PFKL))
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抗原表位
- Center
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PFKL antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 交叉反应
- 牛, 大鼠
- 交叉反应 （详细）
- Rat (95 %), Bovine (94 %)
- 产品特性
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Rabbit Polyclonal antibody to PFKL (phosphofructokinase, liver)
PFKL antibody [C1C3] - 纯化方法
- Purified by antigen-affinity chromatography.
- 免疫原
- Recombinant protein encompassing a sequence within the center region of human PFKL. The exact sequence is proprietary.
- 亚型
- IgG
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anti-Phosphofructokinase, Liver (PFKL) (AA 229-479) antibody
PFKL 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphofructokinase, Liver (PFKL) (AA 700-780) antibodyPFKL 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphofructokinase, Liver (PFKL) (Middle Region) antibodyPFKL 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 绵羊, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Phosphofructokinase, Liver (PFKL) (C-Term) antibodyPFKL 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosphofructokinase, Liver (PFKL) (AA 371-399) antibodyPFKL 适用: 人 WB 宿主: 兔 Polyclonal RB6851 unconjugated
anti-Phosphofructokinase, Liver (PFKL) (C-Term) antibodyPFKL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosphofructokinase, Liver (PFKL) (AA 441-540) antibodyPFKL 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2A9 unconjugated
anti-Phosphofructokinase, Liver (PFKL) (C-Term) antibodyPFKL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphofructokinase, Liver (PFKL) (AA 669-699), (C-Term) antibodyPFKL 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB03903 unconjugated
anti-Phosphofructokinase, Liver (PFKL) (AA 1-827) antibodyPFKL 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
- 说明
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Positive Control: A431 , H1299 , HeLa , HepG2
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.8 mg/mL
- 缓冲液
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- 抗原
- PFKL (Phosphofructokinase, Liver (PFKL))
- 别名
- PFKL (PFKL 产品)
- 别名
- PFK-B antibody, AA407869 antibody, phosphofructokinase, liver type antibody, phosphofructokinase, liver, B-type antibody, PFKL antibody, Pfkl antibody
- 背景
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Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD).
- 分子量
- 85 kDa
- 基因ID
- 5211
- 途径
- Negative Regulation of Hormone Secretion, Warburg Effect
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