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AKR1C1 抗体

Name: AKR1C1 抗体
Brand: antibodies-online
SKU: ABIN2856139
Availability: OutOfStock

KO Validated DDH 适用: 人 WB, IHC, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated

WB Image AKR1C1 antibody detects AKR1C1 protein by Western blot analysis. (AKR1C1 抗体)

WB Image Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody , diluted at 1:1000. (AKR1C1 抗体)

IHC-P Image Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft , using AKR1C1, antibody at 1:500 dilution. (AKR1C1 抗体)

IHC

WB Image Sample(30 ug whole cell lysate) A:Raji , 10% SDS PAGE antibody diluted at 1:1000 (AKR1C1 抗体)

ICC/IF Image Immunofluorescence analysis of methanol-fixed A431, using AKR1C1, antibody at 1:200 dilution. (AKR1C1 抗体)

Liquiritin inhibits progesterone metabolism via AKR1C1. (AKR1C1 抗体)

AKR1C1 is acetylated at Lysines 185 and 201. (AKR1C1 抗体)

Acetylation has no effects on the reductase activity of AKR1C1. (AKR1C1 抗体)

8 images

(3 references)

产品编号 ABIN2856139

发货至: 中国

Datasheet as PDF

抗原 See all AKR1C1 (DDH) 抗体

AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))
适用
36

11

5

2

2

2

2

1

1

1

1
人
宿主
29

7
兔
Compatible Secondaries
克隆类型
32

4
多克隆
标记
25

2

2

1

1

1

1

1

1

1
This AKR1C1 antibody is un-conjugated
应用范围
23

12

11

4

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

交叉反应

人, 小鼠

产品特性

Rabbit Polyclonal antibody to AKR1C1 (aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1, 20-alpha (3-alpha)-hydroxysteroid dehydrogenase))
AKR1C1 antibody

纯化方法

Purified by antigen-affinity chromatography.

质量等级

KO Validated

免疫原

Recombinant protein encompassing a sequence within the center region of human AKR1C1. The exact sequence is proprietary.

亚型

IgG
anti-Dihydrodiol Dehydrogenase (DDH) (AA 1-110) antibody
DDH 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Dihydrodiol Dehydrogenase (DDH) (AA 1-323) antibody
DDH 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Dihydrodiol Dehydrogenase (DDH) (AA 1-323) antibody
DDH 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

anti-Dihydrodiol Dehydrogenase (DDH) (AA 1-110) antibody
DDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B3 unconjugated

anti-Dihydrodiol Dehydrogenase (DDH) antibody
DDH 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal AT6D10 unconjugated

anti-Dihydrodiol Dehydrogenase (DDH) (AA 219-323) antibody
DDH 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Dihydrodiol Dehydrogenase (DDH) antibody
DDH 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Dihydrodiol Dehydrogenase (DDH) (AA 1-323) antibody
DDH 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Dihydrodiol Dehydrogenase (DDH) (N-Term) antibody
DDH 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Dihydrodiol Dehydrogenase (DDH) (N-Term) antibody
DDH 适用: 人, 大鼠, 兔, Cow, 犬, 马, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: mouse liver , A549 , AKR1C1-transfected 293T
Validation: KO/KD, Orthogonal, Overexpression

限制

仅限研究用
状态

Liquid

浓度

0.6 mg/mL

缓冲液

1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Zhu, Hu, Zeng, Chang, Ge, Wang, Yan, Zhao, Cao, Ying, Gu, Zheng, He, Yang: "The SIRT2-mediated deacetylation of AKR1C1 is required for suppressing its pro-metastasis function in Non-Small Cell Lung Cancer." in: Theranostics, Vol. 10, Issue 5, pp. 2188-2200, (2020) (PubMed).

Zeng, Zhu, You, Dong, Yang, Zhu, He: "Liquiritin, as a Natural Inhibitor of AKR1C1, Could Interfere With the Progesterone Metabolism." in: Frontiers in physiology, Vol. 10, pp. 833, (2019) (PubMed).

Zhu, Chang, Yan, Hu, Zeng, Zhou, Yuan, Ying, Cao, He, Yang: "AKR1C1 Activates STAT3 to Promote the Metastasis of Non-Small Cell Lung Cancer." in: Theranostics, Vol. 8, Issue 3, pp. 676-692, (2018) (PubMed).
抗原

AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

别名

aldo-keto reductase family 1 member C1 (DDH 产品)

别名

2-ALPHA-HSD antibody, 20-ALPHA-HSD antibody, C9 antibody, DD1 antibody, DD1/DD2 antibody, DDH antibody, DDH1 antibody, H-37 antibody, HAKRC antibody, HBAB antibody, MBAB antibody, aldo-keto reductase family 1 member C1 antibody, aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase) antibody, AKR1C1 antibody

背景

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Cellular Localization: Cytoplasm

分子量

37 kDa

基因ID

1645

UniProt

Q04828

途径

Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process