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PRKACA 抗体 (Center)

Name: PRKACA 抗体 (Center)
Brand: antibodies-online
SKU: ABIN2855997
Availability: OutOfStock

PRKACA 适用: 人, 小鼠 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated

WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE antibody diluted at 1:1000 (PRKACA 抗体 (Center))

IHC-P Image Immunohistochemical analysis of paraffin-embedded human colon, using PKA alpha, antibody at 1:100 dilution. (PRKACA 抗体 (Center))

IHC

ICC/IF Image Immunofluorescence analysis of methanol-fixed HeLa, using PKA alpha, antibody at 1:200 dilution. (PRKACA 抗体 (Center))

WB Image Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE antibody diluted at 1:1000 (PRKACA 抗体 (Center))

4 images

产品编号 ABIN2855997

发货至: 中国

Datasheet as PDF

抗原 See all PRKACA 抗体

PRKACA (Protein Kinase A, alpha (PRKACA))
抗原表位
15

9

7

6

6

6

4

4

4

3

2

2

1

1

1

1

1

1

1

1

1

1

1
Center
适用
52

35

13

4

4

3

3

2

2

2

2
人, 小鼠
宿主
56

9

2

1
兔
Compatible Secondaries
克隆类型
60

8
多克隆
标记
37

5

4

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This PRKACA antibody is un-conjugated
应用范围
51

31

17

13

13

9

4

3

3

2

2

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

交叉反应

大鼠, 犬, 绵羊, 牛

交叉反应（详细）

Rat (100 %), Dog (100 %), Sheep (100 %), Bovine (100 %)

产品特性

Rabbit Polyclonal antibody to PKA C alpha (protein kinase, cAMP-dependent, catalytic, alpha)
PKA C alpha antibody [N2C3]

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human PKA C alpha. The exact sequence is proprietary.

亚型

IgG
anti-Protein Kinase A, alpha (PRKACA) (AA 233-351), (Catalytic Subunit alpha) antibody
PRKACA 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase A, alpha (PRKACA) (Internal Region) antibody
PRKACA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase A, alpha (PRKACA) (pThr197) antibody
PRKACA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase A, alpha (PRKACA) (AA 1-120) antibody
PRKACA 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 1D7 unconjugated

anti-Protein Kinase A, alpha (PRKACA) (AA 1-120) antibody
PRKACA 适用: 人 ELISA 宿主: 小鼠 Monoclonal 7H3A4 unconjugated

anti-Protein Kinase A, alpha (PRKACA) (AA 1-120) antibody
PRKACA 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Protein Kinase A, alpha (PRKACA) (pSer339) antibody
PRKACA 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase A, alpha (PRKACA) (AA 67-94), (N-Term) antibody
PRKACA 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB20762 unconjugated

anti-Protein Kinase A, alpha (PRKACA) antibody
PRKACA 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase A, alpha (PRKACA) (N-Term) antibody
PRKACA 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

说明

Positive Control: HeLa , Jurkat , NT2D1 , mouse brain

限制

仅限研究用
状态

Liquid

浓度

0.38 mg/mL

缓冲液

0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
抗原

PRKACA (Protein Kinase A, alpha (PRKACA))

别名

PKA C alpha (PRKACA 产品)

别名

PKACA antibody, Calpha antibody, Cs-PKA antibody, PKCA1 antibody, PKA antibody, PKCD antibody, Pkaca antibody, zgc:158799 antibody, protein kinase cAMP-activated catalytic subunit alpha antibody, protein kinase, cAMP dependent, catalytic, alpha antibody, cAMP-dependent protein kinase catalytic subunit antibody, protein kinase, cAMP-dependent, catalytic, alpha, genome duplicate a antibody, PRKACA antibody, Prkaca antibody, kin-1 antibody, prkacaa antibody

背景

CAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Cellular Localization: Cytoplasm (By similarity) , Nucleus (By similarity)

分子量

41 kDa

基因ID

5566

途径

NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, Lipid Metabolism, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection