PD-L1 抗体 (C-Term)
(7 references)-
- 抗原 See all PD-L1 抗体
- PD-L1 (CD274 (PD-L1))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PD-L1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 交叉反应
- 人
- 产品特性
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Rabbit Polyclonal antibody to PD-L1 (CD274 Molecule)
PD-L1 antibody - 纯化方法
- Purified by antigen-affinity chromatography.
- 质量等级
- KO Validated
- 免疫原
- Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PD-L1. The exact sequence is proprietary.
- 亚型
- IgG
- Top Product
- Discover our top product PD-L1 Primary Antibody
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anti-CD274 (PD-L1) (C-Term) antibody
Verified PD-L1 适用: 人 WB, FACS, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 19-238) antibodyPD-L1 适用: 人 WB, FACS, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 9B5F4 unconjugated
anti-CD274 (PD-L1) (AA 39-191) antibodyPD-L1 适用: 人, 小鼠 WB, FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal PDL1-2746 unconjugated
anti-CD274 (PD-L1) (AA 24-153) antibodyPD-L1 适用: 人 WB, FACS, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 6D9A2 unconjugated
anti-CD274 (PD-L1) (AA 48-290) antibodyPD-L1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (Extracellular Domain) antibodyPD-L1 适用: 人 IHC 宿主: 兔 Monoclonal unconjugated
anti-CD274 (PD-L1) (AA 34-241) antibodyPD-L1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 19-238) antibodyPD-L1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 24-153) antibodyPD-L1 适用: 人 WB, FACS, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 7D2A10 unconjugated
anti-CD274 (PD-L1) (AA 201-290) antibodyPD-L1 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- 说明
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Positive Control: MDA-MB-231 (24μg/ml Tunicamycin treatment for 16 hr) , MDA-MB-231 , A431(100 ng/mL IFN-gamma treatment for 48 hr)
Validation: Comparison, KO/KD, Orthogonal, Overexpression
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.29 mg/mL
- 缓冲液
- 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7), 0.025 % ProClin 300
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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: "Differential Expression and Clinicopathological Significance of HER2, Indoleamine 2,3-Dioxygenase and PD-L1 in Urothelial Carcinoma of the Bladder." in: Journal of clinical medicine, Vol. 9, Issue 5, (2020) (PubMed).
: "NPM1 upregulates the transcription of PD-L1 and suppresses T cell activity in triple-negative breast cancer." in: Nature communications, Vol. 11, Issue 1, pp. 1669, (2020) (PubMed).
: "Tumor microenvironment predicts local tumor extensiveness in PD-L1 positive nasopharyngeal cancer." in: PLoS ONE, Vol. 15, Issue 3, pp. e0230449, (2020) (PubMed).
: "Impact of FAK Expression on the Cytotoxic Effects of CIK Therapy in Triple-Negative Breast Cancer." in: Cancers, Vol. 12, Issue 1, (2019) (PubMed).
: "Metformin Promotes Antitumor Immunity via Endoplasmic-Reticulum-Associated Degradation of PD-L1." in: Molecular cell, Vol. 71, Issue 4, pp. 606-620.e7, (2019) (PubMed).
: "Tumor-infiltrating lymphocyte composition, organization and PD-1/ PD-L1 expression are linked in breast cancer." in: Oncoimmunology, Vol. 6, Issue 1, pp. e1257452, (2017) (PubMed).
: "High PD-L1 Expression Correlates with Metastasis and Poor Prognosis in Oral Squamous Cell Carcinoma." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142656, (2016) (PubMed).
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: "Differential Expression and Clinicopathological Significance of HER2, Indoleamine 2,3-Dioxygenase and PD-L1 in Urothelial Carcinoma of the Bladder." in: Journal of clinical medicine, Vol. 9, Issue 5, (2020) (PubMed).
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- 抗原
- PD-L1 (CD274 (PD-L1))
- 别名
- CD274 molecule (PD-L1 产品)
- 别名
- B7-H antibody, B7H1 antibody, PD-L1 antibody, PDCD1L1 antibody, PDCD1LG1 antibody, PDL1 antibody, A530045L16Rik antibody, B7h1 antibody, Pdcd1l1 antibody, Pdcd1lg1 antibody, Pdl1 antibody, RGD1566211 antibody, CD274 molecule antibody, CD274 antigen antibody, programmed cell death 1 ligand 2 antibody, CD274 antibody, Cd274 antibody, PDCD1LG2 antibody
- 背景
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Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Cellular Localization: Isoform 1: Cell membrane, Single-pass type I membrane protein , Isoform 2: Endomembrane system, Single-pass type I membrane protein - 分子量
- 33 kDa
- 基因ID
- 29126
- UniProt
- Q9NZQ7
- 途径
- Cancer Immune Checkpoints
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