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GOLGA5 抗体

Name: GOLGA5 抗体
Brand: antibodies-online
SKU: ABIN2855796
Availability: OutOfStock

GOLGA5 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

WB Image Sample (30 ug of whole cell lysate) A: A549 7. (GOLGA5 抗体)

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GOLGA5, antibody (Green) at 1:500 dilution. (GOLGA5 抗体)

IHC-P Image Immunohistochemical analysis of paraffin-embedded human smooth muscle, using GOLGA5, antibody at 1:100 dilution. (GOLGA5 抗体)

IHC

WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 7. (GOLGA5 抗体)

Fluoxetine induces E-cadherin accumulation in Golgi apparatus, but not ER. (GOLGA5 抗体)

IF (cc)

GPR161 and TMEM220, two novel Golgi-resident proteins involved in protein secretion, regulate glycoprotein secretion by different mechanisms. (GOLGA5 抗体)

IF (cc)

7 images

(4 references)

产品编号 ABIN2855796

发货至: 中国

Datasheet as PDF

抗原 See all GOLGA5 抗体

GOLGA5 (Golgin A5 (GOLGA5))
适用
46

24

23

4

2

1

1

1

1

1

1
人
宿主
46

15
兔
Compatible Secondaries
克隆类型
48

13
多克隆
标记
22

5

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This GOLGA5 antibody is un-conjugated
应用范围
48

21

13

13

13

7

5

4

3

3

2

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

交叉反应

Hamster, 人, 小鼠

产品特性

Rabbit Polyclonal antibody to GOLGA5 (golgin A5)
GOLGA5 antibody [N2C2], Internal

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human GOLGA5. The exact sequence is proprietary.

亚型

IgG
anti-Golgin A5 (GOLGA5) (AA 1-731) antibody
GOLGA5 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Golgin A5 (GOLGA5) (AA 2-99) antibody
GOLGA5 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 6B3 unconjugated

anti-Golgin A5 (GOLGA5) (AA 1-731) antibody
GOLGA5 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Golgin A5 (GOLGA5) (AA 1-300) antibody
GOLGA5 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Golgin A5 (GOLGA5) (AA 1-300) antibody
GOLGA5 适用: 人 WB 宿主: 小鼠 Monoclonal 1720CT766-40-46 unconjugated

anti-Golgin A5 (GOLGA5) (AA 110-159) antibody
GOLGA5 适用: 人, 小鼠, 大鼠, 猴, 豚鼠, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated

anti-Golgin A5 (GOLGA5) (N-Term) antibody
GOLGA5 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Golgin A5 (GOLGA5) (N-Term) antibody
GOLGA5 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, Cow, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated

anti-Golgin A5 (GOLGA5) (AA 401-500) antibody
GOLGA5 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated

anti-Golgin A5 (GOLGA5) (AA 401-500) antibody (Biotin)
GOLGA5 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal Biotin

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: Mouse brain , A549

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Pérez-Rodriguez, de Jesús Ramírez-Lira, Wulff, Voldbor, Ramírez, Trujillo-Roldán, Valdez-Cruz: "Enrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis." in: PLoS ONE, Vol. 15, Issue 8, pp. e0237930, (2020) (PubMed).

Popa, Villeneuve, Stewart, Perez Garcia, Petrunkina Harrison, Moreau: "Genome-wide CRISPR screening identifies new regulators of glycoprotein secretion." in: Wellcome open research, Vol. 4, pp. 119, (2019) (PubMed).

Chang, Chen, Shen, Kung, Chuang, Chen: "Selective serotonin reuptake inhibitor, fluoxetine, impairs E-cadherin-mediated cell adhesion and alters calcium homeostasis in pancreatic beta cells." in: Scientific reports, Vol. 7, Issue 1, pp. 3515, (2019) (PubMed).

Reiling, Olive, Sanyal, Carette, Brummelkamp, Ploegh, Starnbach, Sabatini: "A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens." in: Nature cell biology, Vol. 15, Issue 12, pp. 1473-85, (2013) (PubMed).
抗原

GOLGA5 (Golgin A5 (GOLGA5))

别名

golgin A5 (GOLGA5 产品)

别名

GOLIM5 antibody, RFG5 antibody, ret-II antibody, golim5 antibody, ret-ii antibody, rfg5 antibody, Ret-II antibody, im:7153094 antibody, sb:cb898 antibody, wu:fd50h11 antibody, zgc:66400 antibody, golgin A5 antibody, golgin A5 L homeolog antibody, golgi autoantigen, golgin subfamily a, 5 antibody, GOLGA5 antibody, golga5.L antibody, golga5 antibody, Golga5 antibody

背景

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins, whose members localize to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues, the chimeric sequences have been designated RET-II and PTC5.

Cellular Localization: Golgi apparatus membrane, Single-pass type IV membrane protein

分子量

83 kDa

基因ID

9950

UniProt

Q8TBA6