PRKAA2 抗体 (Center)
(3 references)-
- 抗原 See all PRKAA2 抗体
- PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))
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抗原表位
- Center
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PRKAA2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 交叉反应
- 小鸡, 猪, 大鼠, 牛, Xenopus tropicalis
- 交叉反应 （详细）
- Chicken (96 %), Pig (99 %), Rat (98 %), Bovine (99 %), Xenopus tropicalis (96 %)
- 产品特性
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Rabbit Polyclonal antibody to AMPK alpha 2 (protein kinase, AMP-activated, alpha 2 catalytic subunit)
AMPK alpha 2 antibody - 纯化方法
- Purified by antigen-affinity chromatography.
- 免疫原
- Recombinant protein encompassing a sequence within the center region of human AMPK alpha 2. The exact sequence is proprietary.
- 亚型
- IgG
- Top Product
- Discover our top product PRKAA2 Primary Antibody
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anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (AA 453-552) antibody
PRKAA2 适用: 人 WB, ELISA, IF, RNAi, PLA 宿主: 小鼠 Monoclonal 1G8 unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (AA 453-552) antibodyPRKAA2 适用: 人, 猴 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 8E11H5 unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (AA 351-450) antibodyPRKAA2 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (AA 1-552) antibodyPRKAA2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (AA 350-400), (Catalytic Subunit alpha) antibodyPRKAA2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (AA 265-294) antibodyPRKAA2 适用: 人 WB 宿主: 兔 Polyclonal RB15728 unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (Internal Region) antibodyVerified PRKAA2 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (AA 343-552) antibodyPRKAA2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (pThr172) antibodyPRKAA2 适用: 人 WB, ELISA, IF 宿主: 兔 Monoclonal 4F4 unconjugated Recombinant Antibody
anti-Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (AA 1-552) antibodyPRKAA2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
- 说明
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Positive Control: A431 , HeLa , A375 , NIH-3T3
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.55 mg/mL
- 缓冲液
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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: "Metformin protects against PM2.5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase α2." in: Redox biology, Vol. 28, pp. 101345, (2020) (PubMed).
: "Alternative polyadenylation drives genome-to-phenome information detours in the AMPKα1 and AMPKα2 knockout mice." in: Scientific reports, Vol. 8, Issue 1, pp. 6462, (2018) (PubMed).
: "Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK." in: Nature, Vol. 482, Issue 7384, pp. 251-5, (2012) (PubMed).
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: "Metformin protects against PM2.5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase α2." in: Redox biology, Vol. 28, pp. 101345, (2020) (PubMed).
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- 抗原
- PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))
- 别名
- AMPK alpha 2 (PRKAA2 产品)
- 别名
- AMPK antibody, AMPK2 antibody, AMPKa2 antibody, PRKAA antibody, Ampk antibody, Ampka2 antibody, 2310008I11Rik antibody, A830082D05 antibody, AMPKalpha2 antibody, AMPKA2 antibody, ampk antibody, ampk2 antibody, prkaa antibody, prkaa1 antibody, PRKAA2 antibody, protein kinase AMP-activated catalytic subunit alpha 2 antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit S homeolog antibody, PRKAA2 antibody, Prkaa2 antibody, prkaa2.S antibody, prkaa2 antibody
- 背景
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The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
- 分子量
- 62 kDa
- 基因ID
- 5563
- 途径
- AMPK Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, Warburg Effect
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