Proteasome 26S S3 (Center) 抗体
-
- 抗原
- Proteasome 26S S3
- 抗原表位
- Center
- 适用
- 人, 小鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- 非结合性
-
应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应
- 大鼠
- 交叉反应 (详细)
- Rat (93 %)
- 产品特性
-
Rabbit Polyclonal antibody to Proteasome 26S S3 (proteasome (prosome, macropain) 26S subunit, non-ATPase, 3)
Proteasome 26S S3 antibody [N1N3] - 纯化方法
- Purified by antigen-affinity chromatography.
- 免疫原
- Recombinant protein encompassing a sequence within the center region of human Proteasome 26S S3. The exact sequence is proprietary.
- 亚型
- IgG
-
-
- 应用备注
- Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
- 说明
-
Positive Control: A549 , Hela , Jurkat , NT2D1 , NIH-3T3
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.23 mg/mL
- 缓冲液
- 1XPBS, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
-
- 抗原
- Proteasome 26S S3
- 背景
-
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid.
- 分子量
- 61 kDa
- 基因ID
- 5709
-