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KAP1 抗体

Name: KAP1 抗体
Brand: antibodies-online
SKU: ABIN2855331
Availability: OutOfStock

TRIM28 适用: 人 WB, IF, IP, IHC (p), ICC, ChIP 宿主: 兔 Polyclonal unconjugated

WB Image Sample (30 ug of whole cell lysate) A: H1299 B: Hela 7. (KAP1 抗体)

ICC/IF Image KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunofluorescent analysis. (KAP1 抗体)

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using KAP1, antibody at 1:200 dilution. (KAP1 抗体)

IP Image KAP1 antibody immunoprecipitates KAP1 protein in IP experiments. (KAP1 抗体)

IHC-P Image Immunohistochemical analysis of paraffin-embedded human breast cancer, using KAP1, antibody at 1:500 dilution. (KAP1 抗体)

IHC

ChIP

WB Image Sample (30 ug of whole cell lysate) A:NIH-3T3 7. (KAP1 抗体)

Effects of GRh2 on (A) LPS-induced antioxidative enzymes (catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPx)), heme oxygenase-1 (HO-1), Trx-1, NF-E2-related factor 2 (Nrf2)/Keap1 and KAP1, (B) TLR4, PI3K, Akt, and mTOR, and (C) Raf-1, p-Raf-1, Mek and p-Mek protein expression in the lungs. (KAP1 抗体)

8 images

(4 references)

产品编号 ABIN2855331

发货至: 中国

Datasheet as PDF

抗原 See all KAP1 (TRIM28) 抗体

KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))
适用
100

45

41

10

8

8

7

5

4

2

1

1

1
人
宿主
102

14

4
兔
Compatible Secondaries
克隆类型
97

23
多克隆
标记
60

8

8

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1
This KAP1 antibody is un-conjugated
应用范围
79

37

36

27

26

22

21

20

18

12

6

4

4

3

2

2

2

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

交叉反应

人, 小鼠

产品特性

Rabbit Polyclonal antibody to KAP1 (tripartite motif containing 28)
KAP1 antibody [N3C2], Internal

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human KAP1. The exact sequence is proprietary.

亚型

IgG

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anti-Tripartite Motif Containing 28 (TRIM28) (AA 379-524) antibody
TRIM28 适用: 人 WB, ELISA, IF, IHC (p), RNAi 宿主: 小鼠 Monoclonal 4E6 unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody
Verified TRIM28 适用: 人 ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 576-835) antibody
TRIM28 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (N-Term) antibody
TRIM28 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 379-524) antibody
TRIM28 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1D11 unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 22-291) antibody
TRIM28 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) antibody
TRIM28 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) antibody
TRIM28 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (pSer824) antibody
TRIM28 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 1-835) antibody
TRIM28 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

应用备注

WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: NIH-3T3
Validation: Orthogonal

限制

仅限研究用
状态

Liquid

浓度

0.48 mg/mL

缓冲液

1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Liu, Li, Liu, Li, Wei, Liu, Lv: "Expression and Significance of TRIM 28 in Squamous Carcinoma of Esophagus." in: Pathology oncology research : POR, Vol. 25, Issue 4, pp. 1645-1652, (2020) (PubMed).

Hung, Johnson, Chen, Byrum, Bredemeyer, Yewdell, Johnson, Lee, Deivasigamani, Hindi, Amatya, Gross, Paull, Pisapia, Chaudhuri, Petrini, Mosammaparast, Amarasinghe, Zha, Tyler, Sleckman: "MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining." in: Molecular cell, Vol. 71, Issue 2, pp. 332-342.e8, (2019) (PubMed).

Wang, Wolgemuth: "BET Protein BRDT Complexes With HDAC1, PRMT5, and TRIM28 and Functions in Transcriptional Repression During Spermatogenesis." in: Journal of cellular biochemistry, Vol. 117, Issue 6, pp. 1429-38, (2016) (PubMed).

Aslanian, Yates, Hunter: "Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells." in: DNA repair, Vol. 15, pp. 29-38, (2014) (PubMed).
抗原

KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

别名

tripartite motif containing 28 (TRIM28 产品)

背景

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Cellular Localization: Nucleus (By similarity)

分子量

89 kDa

基因ID

10155

UniProt

Q13263

途径

Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus