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PHGDH 抗体 (N-Term)

Name: PHGDH 抗体 (N-Term)
Brand: antibodies-online
SKU: ABIN2855237
Availability: OutOfStock

KO Validated PHGDH 适用: 人 WB, IHC (p), IF, IP, IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated

WB Image Sample(30 μg of whole cell lysate) A:293T B:A431, C:HeLa S3, 7. (PHGDH 抗体 (N-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using PHGDH, antibody at 1:500 dilution. (PHGDH 抗体 (N-Term))

IHC

ICC/IF Image Immunofluorescence analysis of methanol-fixed HeLa, using PHGDH, antibody at 1:500 dilution. (PHGDH 抗体 (N-Term))

IP Image Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 μg of PHGDH antibody [N1N2], N-term, Western blot analysis was performed using PHGDH antibody [N1N2], N-term, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (PHGDH 抗体 (N-Term))

WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 7. (PHGDH 抗体 (N-Term))

IHC-P Image PHGDH antibody [N1N2], N-term detects PHGDH protein at cytoplasm on mouse pancreas by immunohistochemical analysis. (PHGDH 抗体 (N-Term))

IHC

6 images

产品编号 ABIN2855237

发货至: 中国

Datasheet as PDF

抗原 See all PHGDH 抗体

PHGDH (phosphoglycerate Dehydrogenase (PHGDH))
抗原表位
15

6

6

6

6

5

4

4

2

1

1

1

1

1

1
N-Term
适用
65

15

5

2

2

2

2

1

1

1

1

1

1

1
人
宿主
59

6

1
兔
Compatible Secondaries
克隆类型
61

5
多克隆
标记
26

6

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This PHGDH antibody is un-conjugated
应用范围
50

24

20

13

13

12

10

7

6

3

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

交叉反应

人, 小鼠, 大鼠

产品特性

Rabbit Polyclonal antibody to PHGDH (phosphoglycerate dehydrogenase)
PHGDH antibody [N1N2], N-term

纯化方法

Purified by antigen-affinity chromatography.

质量等级

KO Validated

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human PHGDH. The exact sequence is proprietary.

亚型

IgG

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anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 264-533) antibody
PHGDH 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 140-168) antibody
PHGDH 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 2-251) antibody
PHGDH 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 249-277) antibody
PHGDH 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 1-533) antibody
PHGDH 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 1-533) antibody
PHGDH 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (N-Term) antibody
PHGDH 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 140-168), (N-Term) antibody
PHGDH 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB20882 unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 249-277) antibody
PHGDH 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB20883 unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: Mouse brain , 293T , A431 , HeLaS3 , HeLa
Validation: KO/KD

限制

仅限研究用
状态

Liquid

浓度

0.87 mg/mL

缓冲液

0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
抗原

PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

别名

phosphoglycerate dehydrogenase (PHGDH 产品)

别名

fb38f06 antibody, zgc:65956 antibody, wu:fb38f06 antibody, embryo sac development arrest 9 antibody, F10M10.7 antibody, ECK2909 antibody, JW2880 antibody, 3-PGDH antibody, 3PGDH antibody, 4930479N23 antibody, A10 antibody, PGAD antibody, PGD antibody, PGDH antibody, SERA antibody, PDG antibody, phosphoglycerate dehydrogenase antibody, D-3-phosphoglycerate dehydrogenase antibody, 3-phosphoglycerate dehydrogenase antibody, phgdh antibody, EDA9 antibody, serA antibody, Sthe_2284 antibody, Dacet_1260 antibody, Mrub_0173 antibody, MMAH_RS07650 antibody, Arnit_0487 antibody, Ndas_0174 antibody, Mesil_2386 antibody, Slip_0010 antibody, Trad_0302 antibody, Acear_0027 antibody, Mahau_0665 antibody, MZHIL_RS05165 antibody, Mesop_1331 antibody, Thein_0855 antibody, Phgdh antibody, PHGDH antibody

背景

3-Phosphoglycerate dehydrogenase (PHGDH, EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

分子量

57 kDa

基因ID

26227

UniProt

O43175

途径

Metabolism of Steroid Hormones and Vitamin D, Warburg Effect