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KLF4 抗体 (C-Term)

Name: KLF4 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN2855075
Availability: OutOfStock

KLF4 适用: 人, 小鼠 WB, FACS, IP 宿主: 兔 Polyclonal unconjugated

WB Image Sample (20 ug of whole cell lysate) A: human ESC 10% SDS PAGE antibody diluted at 1:3000 (KLF4 抗体 (C-Term))

IP Image Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 μg of KLF4 antibody, Western blot analysis was performed using KLF4 antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (KLF4 抗体 (C-Term))

WB Image Sample (30 ug of whole cell lysate) A: A549 B: HeLa 10% SDS PAGE antibody diluted at 1:5000 (KLF4 抗体 (C-Term))

FACS

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE antibody diluted at 1:5000 (KLF4 抗体 (C-Term))

WB Image HCT116 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody , diluted at 1:2000. (KLF4 抗体 (C-Term))

WB Image Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE antibody diluted at 1:3000 (KLF4 抗体 (C-Term))

DDX3 suppresses stemness gene signature. (KLF4 抗体 (C-Term))

8 images

(1 reference)

产品编号 ABIN2855075

发货至: 中国

Datasheet as PDF

抗原 See all KLF4 抗体

KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))
抗原表位
11

9

8

7

6

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
100

30

23

9

8

7

7

6

6

5

3

3

2

2

1

1

1

1
人, 小鼠
宿主
82

22

1
兔
Compatible Secondaries
克隆类型
73

32
多克隆
标记
75

6

5

5

4

3

1

1

1

1

1

1

1
This KLF4 antibody is un-conjugated
应用范围
79

49

44

24

23

19

11

9

8

6

2

2

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

交叉反应

大鼠, 猫, 猪, 绵羊, 猕猴, 牛

交叉反应（详细）

Rat (100 %), Cat (100 %), Pig (100 %), Sheep (100 %), Rhesus Monkey (100 %), Bovine (100 %)

产品特性

Rabbit Polyclonal antibody to KLF4 (Kruppel-like factor 4 (gut))
KLF4 antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human KLF4. The exact sequence is proprietary.

亚型

IgG
anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody
KLF4 适用: 人 WB, IF, IHC, IP 宿主: 兔 Monoclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody
KLF4 适用: 人 WB, ELISA, IF, IHC, IP 宿主: 兔 Monoclonal 3A1 unconjugated Recombinant Antibody

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 221-320) antibody
KLF4 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal X1 unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 69-101) antibody
KLF4 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB14263 unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (C-Term) antibody
KLF4 适用: 人, 小鼠, 大鼠, Cow, 马, 犬, 兔, 豚鼠, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 69-101) antibody
KLF4 适用: 人, 小鼠 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 481-504) antibody
KLF4 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody
KO Validated KLF4 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody
KLF4 适用: 人 WB, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 56CT5-1-6 unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 394-421) antibody
KLF4 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated

应用备注

Suggested dilution Reference Flow cytometry/FACS 1:50-1:200* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACS1:50-1:200* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*

说明

Positive Control: A549 , HeLa , HepG2 , HCT116 , NIH-3T3 , human ESC , mouse ESC , HCT116 , HCT116 nuclear extract , *MDM-MB-231

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Nishimura, Okamoto, Shibue, Mizuta, Shibayama, Yoshino, Murakami, Yamaguchi, Tanaka, Toida, Igarashi: "KLF4 is required for suppression of histamine synthesis by polyamines during bone marrow-derived mast cell differentiation." in: PLoS ONE, Vol. 15, Issue 2, pp. e0229744, (2020) (PubMed).
抗原

KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

别名

KLF4 (KLF4 产品)

背景

Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

Cellular Localization: Nucleus

分子量

55 kDa

基因ID

9314

途径

Peptide Hormone Metabolism, Stem Cell Maintenance