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Lactate Dehydrogenase A 抗体

Name: Lactate Dehydrogenase A 抗体
Brand: antibodies-online
SKU: ABIN2855034
Availability: OutOfStock

LDHA 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

WB Image Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHA antibody , diluted at 1:1000. (Lactate Dehydrogenase A 抗体)

ICC/IF Image Immunofluorescence analysis of methanol-fixed A431, using LDHA, antibody at 1:500 dilution. (Lactate Dehydrogenase A 抗体)

WB Image LDHA antibody detects LDHA protein by Western blot analysis. (Lactate Dehydrogenase A 抗体)

ICC/IF Image LDHA antibody detects LDHA protein at cytoplasm by immunofluorescent analysis. (Lactate Dehydrogenase A 抗体)

IHC-P Image Immunohistochemical analysis of paraffin-embedded human lung papillory adenocarcinoma, using LDHA, antibody at 1:500 dilution. (Lactate Dehydrogenase A 抗体)

IHC

8 images

(3 references)

产品编号 ABIN2855034

发货至: 中国

Datasheet as PDF

抗原 See all Lactate Dehydrogenase A (LDHA) 抗体

Lactate Dehydrogenase A (LDHA)
适用
87

43

33

7

7

6

5

4

4

4

3

3

3

2

1

1

1
人
宿主
97

19

5

2
兔
Compatible Secondaries
克隆类型
104

17
多克隆
标记
52

21

15

9

4

4

3

3

3

3

3

3
This Lactate Dehydrogenase A antibody is un-conjugated
应用范围
85

51

47

25

13

11

11

9

6

4

4

4

3

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

交叉反应

人, 小鼠, 大鼠

产品特性

Rabbit Polyclonal antibody to Lactate Dehydrogenase (lactate dehydrogenase A)
LDHA antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human LDHA. The exact sequence is proprietary.

亚型

IgG
anti-Lactate Dehydrogenase A (LDHA) (AA 1-332) antibody
LDHA 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Lactate Dehydrogenase A (LDHA) antibody
LDHA 适用: 人 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 2D11 unconjugated

anti-Lactate Dehydrogenase A (LDHA) (AA 1-332) antibody
LDHA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Lactate Dehydrogenase A (LDHA) (AA 1-332) antibody
LDHA 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Lactate Dehydrogenase A (LDHA) (Middle Region) antibody
LDHA 适用: 人, 小鼠, 大鼠, 兔, Cow, 马, 豚鼠, 犬, 山羊, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Lactate Dehydrogenase A (LDHA) (AA 154-182) antibody
LDHA 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Lactate Dehydrogenase A (LDHA) (AA 204-232) antibody
LDHA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Lactate Dehydrogenase A (LDHA) (AA 5-323) antibody
LDHA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Lactate Dehydrogenase A (LDHA) (C-Term) antibody
LDHA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Lactate Dehydrogenase A (LDHA) antibody
LDHA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: PC-12 , Rat2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , 293T , A431 , HeLa , HepG2
Validation: Orthogonal

限制

仅限研究用
状态

Liquid

浓度

1.33 mg/mL

缓冲液

1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Thompson, Alvarez, Singha, Pavesic, Nguyen, Nelson, Fruman, Razorenova: "Targeting the Mevalonate Pathway Suppresses VHL-Deficient CC-RCC through an HIF-Dependent Mechanism." in: Molecular cancer therapeutics, Vol. 17, Issue 8, pp. 1781-1792, (2019) (PubMed).

Zera, Zastre: "Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes." in: PLoS ONE, Vol. 12, Issue 10, pp. e0186707, (2017) (PubMed).

Ugun-Klusek, Tatham, Elkharaz, Constantin-Teodosiu, Lawler, Mohamed, Paine, Anderson, John Mayer, Lowe, Ellen Billett, Bedford: "Continued 26S proteasome dysfunction in mouse brain cortical neurons impairs autophagy and the Keap1-Nrf2 oxidative defence pathway." in: Cell death & disease, Vol. 8, Issue 1, pp. e2531, (2017) (PubMed).
抗原

Lactate Dehydrogenase A (LDHA)

别名

lactate dehydrogenase A (LDHA 产品)

别名

GSD11 antibody, LDH1 antibody, LDHM antibody, LDH-M antibody, ldh-h antibody, ldha antibody, ldha-B antibody, ldhbb antibody, trg-5 antibody, Ldh1 antibody, Ldhm antibody, l7R2 antibody, gsd11 antibody, ldh-m antibody, ldh1 antibody, ldhm antibody, pig19 antibody, ldha1 antibody, ldhab antibody, LDH-A antibody, LDHC antibody, lactate dehydrogenase A antibody, lactate dehydrogenase B L homeolog antibody, lactate dehydrogenase A4 antibody, lactate dehydrogenase A L homeolog antibody, L-lactate dehydrogenase A chain antibody, LDHA antibody, ldhb.L antibody, Ldha antibody, ldha antibody, ldha.L antibody, LOC100713875 antibody

背景

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

Cellular Localization: Cytoplasm

分子量

37 kDa

基因ID

3939

UniProt

P00338

途径

Warburg Effect