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- 抗原 See all ALDOB 抗体
- ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ALDOB antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 交叉反应
- 人, 小鼠, 大鼠, Turtle
- 产品特性
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Rabbit Polyclonal antibody to Aldolase B (aldolase B, fructose-bisphosphate)
Aldolase B antibody - 纯化方法
- Purified by antigen-affinity chromatography.
- 免疫原
- Recombinant protein encompassing a sequence within the center region of human Aldolase B. The exact sequence is proprietary.
- 亚型
- IgG
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- 应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- 说明
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Positive Control: Neuro2A , GL261 , C8D30 , PC-12 , mouse brain , rat brain , 293T , A431 , HeLa , HepG2
Validation: Orthogonal
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.09 mg/mL
- 缓冲液
- 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Effect of prednisolone on glyoxalase 1 in an inbred mouse model of aristolochic acid nephropathy using a proteomics method with fluorogenic derivatization-liquid chromatography-tandem mass ..." in: PLoS ONE, Vol. 15, Issue 1, pp. e0227838, (2020) (PubMed).
: "Characterization of fructose-1,6-bisphosphate aldolase during anoxia in the tolerant turtle, Trachemys scripta elegans: an assessment of enzyme activity, expression and structure." in: PLoS ONE, Vol. 8, Issue 7, pp. e68830, (2014) (PubMed).
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Effect of prednisolone on glyoxalase 1 in an inbred mouse model of aristolochic acid nephropathy using a proteomics method with fluorogenic derivatization-liquid chromatography-tandem mass ..." in: PLoS ONE, Vol. 15, Issue 1, pp. e0227838, (2020) (PubMed).
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- 抗原
- ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))
- 别名
- aldolase, fructose-bisphosphate B (ALDOB 产品)
- 别名
- aldb antibody, aldo2 antibody, xaldb antibody, ALDOB antibody, aldob antibody, ALDB antibody, ALDO2 antibody, Aldo-2 antibody, Aldo2 antibody, BC016435 antibody, LIV10 antibody, fb25f09 antibody, wu:fb25f09 antibody, aldolase B antibody, aldolase, fructose-bisphosphate B antibody, aldolase, fructose-bisphosphate B S homeolog antibody, aldolase b, fructose-bisphosphate antibody, aldolase B, fructose-bisphosphate antibody, xaldb antibody, aldob antibody, aldob.S antibody, ALDOB antibody, Aldob antibody
- 背景
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Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5 % of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.
- 分子量
- 39 kDa
- 基因ID
- 229
- UniProt
- P05062
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