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VCP 抗体 (C-Term)

Name: VCP 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN2854929
Availability: OutOfStock

KO Validated VCP 适用: 人 WB, IF, IP, IHC (p), ICC, IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated

WB Image VCP antibody detects VCP protein by western blot analysis. (VCP 抗体 (C-Term))

ICC/IF Image VCP antibody detects VCP protein at cytoplasm and nucleus by immunofluorescent analysis. (VCP 抗体 (C-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded human breast cancer, using VCP, antibody at 1:500 dilution. (VCP 抗体 (C-Term))

IHC

IP Image Immunoprecipitation of VCP protein from HeLa whole cell extracts using 5 μg of VCP antibody, Western blot analysis was performed using VCP antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (VCP 抗体 (C-Term))

WB Image Sample(30 μg of whole cell lysate) A:A431, B:H1299 7. (VCP 抗体 (C-Term))

WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 7. (VCP 抗体 (C-Term))

Study of cellular SIL1 immunoreactivity and presence of ER-stress in Hek293 cells overexpressing SIL1 or its variant forms. (VCP 抗体 (C-Term))

8 images

(1 reference)

产品编号 ABIN2854929

发货至: 中国

Datasheet as PDF

抗原 See all VCP 抗体

VCP (Valosin Containing Protein (VCP))
抗原表位
16

10

9

8

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
92

47

39

14

5

5

5

4

4

3

2

2

2

2

1

1

1
人
宿主
78

17

2
兔
Compatible Secondaries
克隆类型
71

26
多克隆
标记
56

8

7

4

3

3

3

3

3

3

2

2
This VCP antibody is un-conjugated
应用范围
64

40

23

20

15

12

11

10

7

2

2

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Whole Mount) (IHC (wm))

特异性

Knockdown/Knockout validation was supported by customer review data.

交叉反应

人, 小鼠, 大鼠, 斑马鱼

产品特性

Rabbit Polyclonal antibody to VCP (valosin containing protein)
VCP antibody

纯化方法

Purified by antigen-affinity chromatography.

质量等级

KO Validated

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human VCP. The exact sequence is proprietary.

亚型

IgG

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anti-Valosin Containing Protein (VCP) (AA 125-371) antibody
VCP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

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VCP 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 4A8 unconjugated

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VCP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

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VCP 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Monoclonal 5H12 unconjugated Recombinant Antibody

anti-Valosin Containing Protein (VCP) antibody
VCP 适用: 人, 小鼠 WB, FACS, ICC 宿主: 小鼠 Monoclonal Hs-14 unconjugated

anti-Valosin Containing Protein (VCP) (AA 732-760), (C-Term) antibody
VCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 749-766), (C-Term) antibody
VCP 适用: 人, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 732-760) antibody
VCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (C-Term) antibody
VCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: Mouse brain , Rat brain
Validation: KO/KD, Orthogonal

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Chen, Morales, Guerrero, Sun, McCarty: "PTPN12/PTP-PEST Regulates Phosphorylation-Dependent Ubiquitination and Stability of Focal Adhesion Substrates in Invasive Glioblastoma Cells." in: Cancer research, Vol. 78, Issue 14, pp. 3809-3822, (2019) (PubMed).
抗原

VCP (Valosin Containing Protein (VCP))

别名

valosin containing protein (VCP 产品)

别名

ALS14 antibody, IBMPFD antibody, TERA antibody, p97 antibody, CDC48 antibody, wu:fd16d05 antibody, wu:fj63d11 antibody, p93 antibody, 3110001E05 antibody, p97/VCP antibody, 22.26 antibody, 7.12 antibody, Apple antibody, BcDNA.GM02885 antibody, BcDNA:GM02885 antibody, CG2331 antibody, DmTER94 antibody, Dmel\CG2331 antibody, I antibody, Ter94 antibody, Tera antibody, VCP antibody, VCP ATPase antibody, VCP/p97 antibody, Vcp antibody, anon-WO2004063362.65 antibody, anon-WO2004063362.67 antibody, cdc48 antibody, dTERA antibody, dVCP antibody, dmTERA antibody, dvcp antibody, l(2)03775 antibody, l(2)46CFf antibody, l(2)46CFs antibody, l(2)46Ch antibody, pTER94 antibody, ter94 antibody, vcp antibody, FANCG antibody, valosin containing protein antibody, valosin containing protein S homeolog antibody, valosin-containing protein antibody, CG2331 gene product from transcript CG2331-RA antibody, zgc:136908 antibody, transitional endoplasmic reticulum ATPase antibody, VCP antibody, vcp.S antibody, vcp antibody, Vcp antibody, Bm1_56585 antibody, LOC100136246 antibody, TER94 antibody, zgc:136908 antibody, LOC101109680 antibody

背景

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.

Cellular Localization: Cytoplasm , cytosol , Nucleus

分子量

89 kDa

基因ID

7415

UniProt

P55072

途径

ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway