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VCP 抗体 (C-Term)

Name: VCP 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN2854929
Availability: OutOfStock

KO Validated VCP 适用: 人 WB, IF, IP, IHC (p), ICC, IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated

WB Image VCP antibody detects VCP protein by western blot analysis. (VCP 抗体 (C-Term))

ICC/IF Image VCP antibody detects VCP protein at cytoplasm and nucleus by immunofluorescent analysis. (VCP 抗体 (C-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded human breast cancer, using VCP, antibody at 1:500 dilution. (VCP 抗体 (C-Term))

IHC

IP Image Immunoprecipitation of VCP protein from HeLa whole cell extracts using 5 μg of VCP antibody, Western blot analysis was performed using VCP antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (VCP 抗体 (C-Term))

WB Image Sample(30 μg of whole cell lysate) A:A431, B:H1299 7. (VCP 抗体 (C-Term))

WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 7. (VCP 抗体 (C-Term))

Study of cellular SIL1 immunoreactivity and presence of ER-stress in Hek293 cells overexpressing SIL1 or its variant forms. (VCP 抗体 (C-Term))

8 images

(1 reference)

产品编号 ABIN2854929

发货至: 中国

Datasheet as PDF

抗原 See all VCP 抗体

VCP (Valosin Containing Protein (VCP))
抗原表位
11

10

9

8

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
88

47

39

14

5

5

5

4

4

3

2

2

2

2

1

1

1
人
宿主
74

17

2
兔
Compatible Secondaries
克隆类型
66

27
多克隆
标记
57

7

6

3

3

3

3

3

3

3

1

1
This VCP antibody is un-conjugated
应用范围
60

36

24

20

16

12

11

11

7

2

2

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Whole Mount) (IHC (wm))

特异性

Knockdown/Knockout validation was supported by customer review data.

交叉反应

人, 小鼠, 大鼠, 斑马鱼

产品特性

Rabbit Polyclonal antibody to VCP (valosin containing protein)
VCP antibody

纯化方法

Purified by antigen-affinity chromatography.

质量等级

KO Validated

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human VCP. The exact sequence is proprietary.

亚型

IgG

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VCP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

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VCP 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Monoclonal 5H12 unconjugated Recombinant Antibody

anti-Valosin Containing Protein (VCP) antibody
VCP 适用: 人, 小鼠 WB, ICC, FACS 宿主: 小鼠 Monoclonal Hs-14 unconjugated

anti-Valosin Containing Protein (VCP) (AA 732-760), (C-Term) antibody
VCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 749-766), (C-Term) antibody
VCP 适用: 人, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 732-760) antibody
VCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (C-Term) antibody
VCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: Mouse brain , Rat brain
Validation: KO/KD, Orthogonal

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Chen, Morales, Guerrero, Sun, McCarty: "PTPN12/PTP-PEST Regulates Phosphorylation-Dependent Ubiquitination and Stability of Focal Adhesion Substrates in Invasive Glioblastoma Cells." in: Cancer research, Vol. 78, Issue 14, pp. 3809-3822, (2019) (PubMed).
抗原

VCP (Valosin Containing Protein (VCP))

别名

valosin containing protein (VCP 产品)

背景

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.

Cellular Localization: Cytoplasm , cytosol , Nucleus

分子量

89 kDa

基因ID

7415

UniProt

P55072

途径

ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway