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ALPL 抗体 (Center)

Name: ALPL 抗体 (Center)
Brand: antibodies-online
SKU: ABIN2854870
Availability: OutOfStock

ALPL 适用: 人, 小鼠, 绵羊 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

WB Image Sample (30 ug of whole cell lysate) A: Hela 7. (ALPL 抗体 (Center))

IHC-P Image Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using alkaline phosphatase (liver/bone/kidney), antibody at 1:100 dilution. (ALPL 抗体 (Center))

IHC

2 images

(1 reference)

产品编号 ABIN2854870

发货至: 中国

Datasheet as PDF

抗原 See all ALPL 抗体

ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))
抗原表位
16

9

9

5

5

4

4

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
Center
适用
81

48

17

5

1

1

1

1
人, 小鼠, 绵羊
宿主
78

19

1

1
兔
Compatible Secondaries
克隆类型
81

18
多克隆
标记
56

9

6

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This ALPL antibody is un-conjugated
应用范围
65

38

26

14

13

13

11

11

8

6

5

3

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

交叉反应

小鼠, 猫, 犬, 大鼠, 牛

交叉反应（详细）

Mouse (91 %), Cat (90 %), Dog (89 %), Rat (91 %), Bovine (89 %)

产品特性

Rabbit polyclonal antibody to alkaline phosphatase (liver/bone/kidney) (alkaline phosphatase, liver/bone/kidney)
Alkaline Phosphatase (Tissue Non-Specific) antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human Alkaline Phosphatase (Tissue Non-Specific). The exact sequence is proprietary.

亚型

IgG

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anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 18-335) antibody
ALPL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) antibody
ALPL 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 2F4 unconjugated

anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) antibody
ALPL 适用: 人, Cow IHC, StM 宿主: 小鼠 Monoclonal ALPL-597 unconjugated

anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 21-35), (N-Term) antibody
ALPL 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 217-246) antibody
ALPL 适用: 人, 小鼠 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated

anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 91-200) antibody
ALPL 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 4H1 unconjugated

anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (Internal Region) antibody
ALPL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 20-270) antibody
ALPL 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) antibody
ALPL 适用: 人 WB, IHC (p), FACS, EIA 宿主: 小鼠 Monoclonal 2F4 unconjugated

anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 52-150) antibody
ALPL 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

Suggested dilution Reference Immunohistochemistry Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceImmunohistochemistryAssay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

说明

Positive Control: HeLa , HepG2 , Molt-4 , Raji

限制

仅限研究用
状态

Liquid

浓度

0.48 mg/mL

缓冲液

0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Agustina, Asyifa, Aziz, Hernowo: "The Role of Osteocalcin and Alkaline Phosphatase Immunohistochemistry in Osteosarcoma Diagnosis." in: Pathology research international, Vol. 2018, pp. 6346409, (2018) (PubMed).
抗原

ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

别名

Alkaline Phosphatase (Liver/bone/kidney) (ALPL 产品)

别名

ALP antibody, iap antibody, alpl antibody, AP-TNAP antibody, APTNAP antibody, HOPS antibody, TNAP antibody, TNSALP antibody, Akp-2 antibody, Akp2 antibody, PHOA antibody, TNS-AP antibody, alkaline phosphatase, liver/bone/kidney L homeolog antibody, alkaline phosphatase, liver/bone/kidney antibody, alkaline phosphatase, intestinal, gene 2 antibody, alkaline phosphatase, liver/bone/kidney pseudogene antibody, alpl.L antibody, ALPL antibody, alpi.2 antibody, LOC100031702 antibody, Alpl antibody

背景

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Cellular Localization: Cell membrane, Lipid-anchor , GPI-anchor

分子量

57 kDa

基因ID

249