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Vimentin 抗体

Name: Vimentin 抗体
Brand: antibodies-online
SKU: ABIN2854814
Availability: OutOfStock

KO Validated VIM 适用: 人 WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated

WB Image Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody , diluted at 1:10000. (Vimentin 抗体)

WB Image Vimentin antibody detects Vimentin protein by western blot analysis. (Vimentin 抗体)

IHC-P Image Vimentin antibody detects Vimentin protein at cytoplasm in rat testis by immunohistochemical analysis. (Vimentin 抗体)

IHC

IP Image Immunoprecipitation of Vimentin protein from HeLa whole cell extracts using 5 μg of Vimentin antibody, Western blot analysis was performed using Vimentin antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (Vimentin 抗体)

IHC-P Image Vimentin antibody detects Vimentin protein at cytosol on mouse thymus gland by immunohistochemical analysis. (Vimentin 抗体)

IHC

IHC-Fr Image Immunofluorescence photomicrographs of frozen sections of mouse brain. (Vimentin 抗体)

IHC

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Vimentin, antibody (Green) at 1:500 dilution. (Vimentin 抗体)

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Vimentin, antibody at 1:500 dilution. (Vimentin 抗体)

IHC

Effects of MCME on migration-related proteins in CL1-0 and CL1-5 cells. (Vimentin 抗体)

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin. (Vimentin 抗体)

Fusion progeny exhibit epithelial-mesenchymal transition(A) Representative phase-contrast microscopic images of fusion progeny and parental cells. (Vimentin 抗体)

FOXF1 mediates reprogramming effects on fusion progeny(A) FOXF1 mRNA levels were determined by real-time PCR in fusion progeny and parent cell lines. (Vimentin 抗体)

Expression pattern of Notch ligands and receptors in first trimester decidual tissue. (Vimentin 抗体)

IF (cc)

MEF2B-V5 expression alters the abundance of protein from target genes and increases HEK293A cell migration. (Vimentin 抗体)

MEF2B mutations alter the abundance of protein from MEF2B target genes and decrease cell migration. (Vimentin 抗体)

Serum-free suspension culture induces the enrichment of Glioma stem-like cells (GSCs). (Vimentin 抗体)

Aryl hydrocarbon receptor (AhR) expression in different cells was correlated with E-cadherin and vimentin expression and cell motility. (Vimentin 抗体)

Level of autophagy-related protein expression in different cells was correlated with their cell motility. (Vimentin 抗体)

Expression of epithelial-mesenchymal transition (EMT) markers in MCF-7 o/e Snail cells. (Vimentin 抗体)

Differential expression of epithelial-mesenchymal transition (EMT) markers in MCF-7 o/e Snail cells and MCF-F o/e Snail tissues. (Vimentin 抗体)

ZEB2-AS1 knockdown inhibits migration, invasion and EMT in HNSCC cells. (Vimentin 抗体)

Reintroduction of exogenous ZEB2 largely attenuated the phenotypical changes induced by ZEB2-AS1 inhibition. (Vimentin 抗体)

ZEB2-AS1 is involved for TGF-β1-induced EMT in HNSCC cells. (Vimentin 抗体)

Lipid loading augments hypoxia mediated secretion of pro-tumorigenic factors. (Vimentin 抗体)

ISX expression induces TWIST1 and Snail1 expression, and promotes EMTAThe expression levels of ISX, TWIST1, Snail1, fibronectin (FN1), VEGF, and E-cadherin (CDH1) mRNA were examined in a ISX-GFP inducible Tet-ON transformants at 8 h after addition of DOX. (Vimentin 抗体)

ISX interacts with both BRD4 and PCAF in vitro and in vivoABRD4 peptide (291-314 a. (Vimentin 抗体)

The expression of ISX, BRD4, and PCAF is correlated with lung cancer metastasisAIHC staining with ISX (brown) or PCAF (brown) antibody in lung tumors from patients with lung cancer. (Vimentin 抗体)

Sequencing analysis of acetylated recombinant and trypsin-digested wild-type ISX using liquid chromatography-mass spectrometryA, BPCAF acetylation of ISX was detected at lysine residue 69 by liquid chromatography-tandem mass spectrometry (LC-tandem MS-MS). (Vimentin 抗体)

Cataracts induced epithelial-mesenchymal transition (EMT) progression with significantly elevated N-cadherin and MMP9 expression in DM (+) patients. (Vimentin 抗体)

IF (cc)

35 images

(17 references)

产品编号 ABIN2854814

发货至: 中国

Datasheet as PDF

抗原 See all Vimentin (VIM) 抗体

Vimentin (VIM)
适用
341

134

131

60

60

49

42

37

29

21

20

13

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
人
宿主
194

130

11

6

6

5

2
兔
Compatible Secondaries
克隆类型
210

143
多克隆
标记
203

19

14

14

10

7

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
应用范围
285

130

105

99

95

63

45

40

39

32

23

18

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

Cat, 犬, 人, 小鼠, 大鼠

产品特性

Rabbit Polyclonal antibody to Vimentin (vimentin)
Vimentin antibody

纯化方法

Purified by antigen-affinity chromatography.

质量等级

KO Validated

免疫原

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

亚型

IgG

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VIM 适用: 人, 小鼠, 大鼠, Pig, Cow, 猴 WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 哺乳动物 WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, 小鼠, 大鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C8 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 山羊 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal VM452 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody

anti-Vimentin (VIM) (AA 210-466) antibody
VIM 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D7 unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

应用备注

WB: 1:5000-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: 293T
Validation: Comparison, KO/KD, Orthogonal, Overexpression

限制

仅限研究用
状态

Liquid

浓度

0.11 mg/mL

缓冲液

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Navarrete-Bernal, Cervantes-Badillo, Martínez-Herrera, Lara-Torres, Gerson-Cwilich, Zentella-Dehesa, Ibarra-Sánchez, Esparza-López, Montesinos, Cortés-Morales, Osorio-Pérez, Villegas-Osorno et al.: "Biological Landscape of Triple Negative Breast Cancers Expressing CTLA-4. ..." in: Frontiers in oncology, Vol. 10, pp. 1206, (2020) (PubMed).

Klein, Lohr, Droste: "Age-Dependent Heterogeneity of Murine Olfactory Bulb Astrocytes." in: Frontiers in aging neuroscience, Vol. 12, pp. 172, (2020) (PubMed).

Horn, Riskin, Hempel, Fousek, Lind, Hamilton, McCampbell, Maeda, Zebala, Su, Schlom, Palena: "Simultaneous inhibition of CXCR1/2, TGF-β, and PD-L1 remodels the tumor and its microenvironment to drive antitumor immunity." in: Journal for immunotherapy of cancer, Vol. 8, Issue 1, (2020) (PubMed).

Wang, Liu, Jeng, Chiang, Chai, Chiou, Huang, Yokoyama, Wang, Huang, Hsu: "PCAF-mediated acetylation of ISX recruits BRD4 to promote epithelial-mesenchymal transition." in: EMBO reports, Vol. 21, Issue 2, pp. e48795, (2020) (PubMed).

Diao, Ge, Song, Wu, Li, Li, Yang, Wang, Cheng: "Overexpression of ZEB2-AS1 promotes epithelial-to-mesenchymal transition and metastasis by stabilizing ZEB2 mRNA in head neck squamous cell carcinoma." in: Journal of cellular and molecular medicine, Vol. 23, Issue 6, pp. 4269-4280, (2020) (PubMed).

Xie, Zhao, Guo, Liu, Fu, Fan, Lin, Chen, Wang, Fan, Wei: "Benzyl isothiocyanate suppresses development and metastasis of murine mammary carcinoma by regulating the Wnt/β‑catenin pathway." in: Molecular medicine reports, Vol. 20, Issue 2, pp. 1808-1818, (2019) (PubMed).

Offer, Menard, Pérez, de Oliveira, Chandran, Johansson, Bång-Rudenstam, Siesjö, Ebbesson, Hedenfalk, Sundgren, Darabi, Belting: "Extracellular lipid loading augments hypoxic paracrine signaling and promotes glioma angiogenesis and macrophage infiltration." in: Journal of experimental & clinical cancer research : CR, Vol. 38, Issue 1, pp. 241, (2019) (PubMed).

Hua, Liu, Hsu, Cheng, Yang, Chang, Lee, Hsiao, Juan, Chien, Yang: "3'UTR polymorphisms of carbonic anhydrase IX determine the miR-34a targeting efficiency and prognosis of hepatocellular carcinoma." in: Scientific reports, Vol. 7, Issue 1, pp. 4466, (2019) (PubMed).

Chu, Lee, Lee, Wang, Huang, Lan, Lin, Lin, Tian, Liu, Chow: "MiR-338-5p promotes metastasis of colorectal cancer by inhibition of phosphatidylinositol 3-kinase, catalytic subunit type 3-mediated autophagy pathway." in: EBioMedicine, Vol. 43, pp. 270-281, (2019) (PubMed).

Nakazawa, Taniyama, Sanada, Morishita, Nakamori, Morimoto, Yeung, Yang: "Periostin blockade overcomes chemoresistance via restricting the expansion of mesenchymal tumor subpopulations in breast cancer." in: Scientific reports, Vol. 8, Issue 1, pp. 4013, (2019) (PubMed).

Chiu, Wen, Wang, Hsu, Tsai, Hung, Tseng, Shyr: "Role of estrogen receptors and Src signaling in mechanisms of bone metastasis by estrogen receptor positive breast cancers." in: Journal of translational medicine, Vol. 15, Issue 1, pp. 97, (2018) (PubMed).

Wang, Wu, Yeh, Su, Hsia, Liang, Chen, Hsueh, Chen: "Melatonin suppresses hepatocellular carcinoma progression via lncRNA-CPS1-IT-mediated HIF-1α inactivation." in: Oncotarget, Vol. 8, Issue 47, pp. 82280-82293, (2017) (PubMed).

Hsu, Chang, Hsu, Liu, Yeh, Wang, Liou, Ko, Chang, Chuang: "Suberoylanilide hydroxamic acid represses glioma stem-like cells." in: Journal of biomedical science, Vol. 23, Issue 1, pp. 81, (2017) (PubMed).

Yin, Yin, Wang, Zhou, Liu, Yang, Wang, Lu: "MiR-206 suppresses epithelial mesenchymal transition by targeting TGF-β signaling in estrogen receptor positive breast cancer cells." in: Oncotarget, Vol. 7, Issue 17, pp. 24537-48, (2017) (PubMed).

Marquez-Vilendrer, Rai, Gramling, Lu, Reisman: "Loss of the SWI/SNF ATPase subunits BRM and BRG1 drives lung cancer development." in: Oncoscience, Vol. 3, Issue 11-12, pp. 322-336, (2016) (PubMed).

Hou, You, Yang, Pan, Chen, Lee, Lin, Liou, Liu, Chi, Ger, Tsai: "Aberrant DNA hypomethylation of miR-196b contributes to migration and invasion of oral cancer." in: Oncology letters, Vol. 11, Issue 6, pp. 4013-4021, (2016) (PubMed).

Wei, Nickoloff, Chen, Liu, Lo, Chang, Yang, Wu, Williams, Gelovani, Deng: "FOXF1 mediates mesenchymal stem cell fusion-induced reprogramming of lung cancer cells." in: Oncotarget, Vol. 5, Issue 19, pp. 9514-29, (2015) (PubMed).
抗原

Vimentin (VIM)

别名

vimentin (VIM 产品)

别名

CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

背景

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

分子量

54 kDa

基因ID

7431

UniProt

P08670

途径

Caspase Cascade in Apoptosis