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beta Actin 抗体 (N-Term)

Name: beta Actin 抗体 (N-Term)
Brand: antibodies-online
SKU: ABIN2854709
Availability: OutOfStock

KO Validated ACTB 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

WB Image Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE Beta-actin antibody antibody diluted at 1:1000 (beta Actin 抗体 (N-Term))

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin, antibody (Green) at 1:1000 dilution. (beta Actin 抗体 (N-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Actin, antibody at 1:500 dilution. (beta Actin 抗体 (N-Term))

IHC

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE antibody diluted at 1:5000 (beta Actin 抗体 (N-Term))

WB Image Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody , diluted at 1:1000. (beta Actin 抗体 (N-Term))

IHC-P Image beta Actin antibody detects beta Actin protein at cytosol on mouse prostate by immunohistochemical analysis. (beta Actin 抗体 (N-Term))

IHC

6 images

(4 references)

产品编号 ABIN2854709

发货至: 中国

Datasheet as PDF

抗原 See all beta Actin (ACTB) 抗体

beta Actin (ACTB) (Actin, beta (ACTB))
抗原表位
40

16

15

6

6

5

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
181

131

111

55

42

42

37

27

25

23

18

17

12

10

8

7

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1
人
宿主
115

82

5

1

1
兔
Compatible Secondaries
克隆类型
112

90
多克隆
标记
129

13

12

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is un-conjugated
应用范围
171

74

54

46

32

30

26

22

13

13

8

6

6

6

5

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

交叉反应

Cat, 犬, Drosophila melanogaster, 人, 小鼠

产品特性

Rabbit Polyclonal antibody to beta Actin (actin, beta)
beta Actin antibody

纯化方法

Purified by antigen-affinity chromatography.

质量等级

KO Validated

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human beta Actin. The exact sequence is proprietary.

亚型

IgG

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anti-Actin, beta (ACTB) (AA 1-375) antibody
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anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 小鸡, 兔, Pig, Cow, Hamster, 山羊, 鱼 WB, ELISA 宿主: 小鼠 Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), FACS, DNA Mic 宿主: 小鼠 Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人 WB, ELISA, IHC, IF, IP, FACS 宿主: 小鼠 Monoclonal 1D12E12B5 unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: NIH-3T3 , Raji , 293T
Validation: KO/KD

限制

仅限研究用
状态

Liquid

浓度

1.16 mg/mL

缓冲液

1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Yu, Luan, Liu, Su, Li: "MiR-296 Promotes Osteoblast Differentiation by Upregulating Cbfal." in: Pharmacology, Vol. 105, Issue 3-4, pp. 190-201, (2020) (PubMed).

Tao, Cheng, Yang: "Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway." in: Clinical interventions in aging, Vol. 15, pp. 373-385, (2020) (PubMed).

Cai, Zhang, Zhang, Chen, Hu: "Effect of Th9/IL-9 on the growth of gastric cancer in nude mice." in: OncoTargets and therapy, Vol. 12, pp. 2225-2234, (2019) (PubMed).

Li, Yu, Jiao, Wang, Zhang, Sun: "Vandetanib (ZD6474) induces antiangiogenesis through mTOR-HIF-1 alpha-VEGF signaling axis in breast cancer cells." in: OncoTargets and therapy, Vol. 11, pp. 8543-8553, (2018) (PubMed).
抗原

beta Actin (ACTB) (Actin, beta (ACTB))

别名

actin beta (ACTB 产品)

背景

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Cellular Localization: Cytoplasm , cytoskeleton

分子量

42 kDa

基因ID

60

UniProt

P60709

途径

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction