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Cortactin 抗体 (N-Term)

Name: Cortactin 抗体 (N-Term)
Brand: antibodies-online
SKU: ABIN2854674
Availability: OutOfStock
Rating: 5 (1 reviews)

KO Validated CTTN 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

Multiplex Immunohistochemistry validation image for anti-Cortactin (CTTN) (N-Term) antibody (ABIN2854674) (Cortactin 抗体 (N-Term))

mIHC

WB Image Sample (30 ug of whole cell lysate) A: PC-12 7. (Cortactin 抗体 (N-Term))

WB Image Sample (30 ug of whole cell lysate) A: Hep G2 , 7. (Cortactin 抗体 (N-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded human breast cancer, using Cortactin, antibody at 1:250 dilution. (Cortactin 抗体 (N-Term))

IHC

ICC/IF Image Cortactin antibody [N1], N-term detects Cortactin protein at cell membrane and cytoplasm by immunofluorescent analysis. (Cortactin 抗体 (N-Term))

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. (Cortactin 抗体 (N-Term))

7 images

(1 validation)

产品编号 ABIN2854674

发货至: 中国

Datasheet as PDF

抗原 See all Cortactin (CTTN) 抗体

Cortactin (CTTN)
抗原表位
15

14

12

7

7

7

6

6

6

5

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
126

62

44

5

4

2

2

2

2

2

2

1

1

1
人
宿主
126

6

1
兔
Compatible Secondaries
克隆类型
118

15
多克隆
标记
76

8

4

4

4

4

4

4

4

4

3

3

1

1

1

1

1

1

1

1

1

1

1
This Cortactin antibody is un-conjugated
应用范围
98

56

54

16

15

14

14

12

10

5

3

3

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

交叉反应

人, 小鼠, 大鼠

产品特性

Rabbit Polyclonal antibody to Cortactin (src substrate cortactin)
Cortactin antibody [N1], N-term

纯化方法

Purified by antigen-affinity chromatography.

质量等级

KO Validated

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Cortactin. The exact sequence is proprietary.

亚型

IgG
anti-Cortactin (CTTN) (AA 214-513) antibody
CTTN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Cortactin (CTTN) antibody
CTTN 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Cortactin (CTTN) (AA 341-450) antibody
CTTN 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2B5 unconjugated

anti-Cortactin (CTTN) (pTyr466) antibody
CTTN 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cortactin (CTTN) antibody
CTTN 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4C6 unconjugated

anti-Cortactin (CTTN) (AA 1-509) antibody
CTTN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cortactin (CTTN) (AA 1-509) antibody
CTTN 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cortactin (CTTN) (pTyr421) antibody
CTTN 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cortactin (CTTN) (N-Term) antibody
Verified CTTN 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Cortactin (CTTN) (AA 1-200) antibody
CTTN 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: NIH-3T3 , JC , PC-12 , mouse brain , rat brain , HeLa
Validation: KO/KD

限制

仅限研究用
生效 #104334 (Multiplex Immunohistochemistry)

by

Akoya Biosciences

No.

#104334

日期

2021.04.20

抗原

Cortactin

Lot Number

39568

Method validated

Multiplex Immunohistochemistry

Positive Control

Fresh frozen mouse olfactory bulb

Negative Control

Unlabeled control (mouse fresh frozen)

Notes

Passed. The anti-cortactin antibody ABIN2854674 produces high degrees of labeling in granular and external plexiform layers of the mouse olfactory bulb. Additional labeling can be observed in the neuropil of individual glomeruli which is consistent with staining of synapses and neuronal processes.

Validation Images

Murine fresh frozen coronal olfactory bulb section (Thickness = 5 µm) labeled with anti-cortactin antibody ABIN2854674 (green; conjugated to fluorophore ATTO 550). Labeling is present in high degrees of labeling in granular and external plexiform layers with additional staining observed in the glomerular neuropil regions. Grin2b and doublecortin were labeled with ABIN5611338 (red; bound to fluorophore ATTO 550) and a doublecortin antibody (blue; bound to fluorophore AF 488).

FFPE normal human cortex tissue section labeled with anti-cortactin antibody ABIN2854674 (green; bound to fluorophore ATTO 550) after EDTA antigen retrieval. MAP2 and neurofilament were additionally labeled with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 550) and anti-neurofilament antibody (blue; bound to fluorophore AF488).
Full Methods
Primary Antibody

ABIN2854674

Secondary Antibody

Full Protocol

Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
Conjugation of the anti-cortactin ABIN2854674 antibody to an oligo barcode used to bind oligo-conjugated fluorophore ATTO 550.

Experimental Notes

No signal was detected in unlabeled specimens.
Specific staining of Cortactin was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.
Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).
状态

Liquid

浓度

1 mg/mL

缓冲液

1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
抗原

Cortactin (CTTN)

别名

cortactin (CTTN 产品)

别名

EMS1 antibody, 1110020L01Rik antibody, Ems1 antibody, Cttnb antibody, CG3637 antibody, Dmel\CG3637 antibody, cortactin antibody, cttn antibody, CTTN1 antibody, P85.25 antibody, Cttn antibody, ems1 antibody, CTTN antibody, cortactin antibody, CG3637 gene product from transcript CG3637-RD antibody, cortactin S homeolog antibody, cortactin L homeolog antibody, src substrate cortactin antibody, Src substrate cortactin antibody, CTTN antibody, Cttn antibody, Cortactin antibody, cttn.S antibody, cttn.L antibody, cttn antibody, CpipJ_CPIJ006351 antibody, Bm1_57220 antibody, LOC100533277 antibody

背景

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.

Cellular Localization: Cytoplasm , cytoskeleton (By similarity) , Cell projection , lamellipodium (By similarity) , ruffle (By similarity)

分子量

62 kDa

基因ID

2017

UniProt

Q14247

途径

MAPK Pathway