BrdU 抗体
(10 references)Quick Overview for BrdU 抗体 (ABIN285018)
抗原
See all BrdU 抗体适用
宿主
克隆类型
标记
应用范围
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交叉反应 （详细）
- Not species specific. Nucleotide is present in all species. Very low cross reactivity to 5-methyl cytosine.
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纯化方法
- purified
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免疫原
- BRDU antibody was raised in sheep using BrdU coupled to KLH as the immunogen.
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anti-Bromodeoxyuridine (BrdU) antibody
BrdU FACS, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal Bu20a unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), IF (cc), IF (p), IHC (fro), WB 宿主: 小鼠 Monoclonal A7 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), IF (cc), IF (p), IHC (fro), ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, FACS, IF, WB, ELISA, FM, DB 宿主: 小鼠 Monoclonal 29G6-E8 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, Neut, StM 宿主: 小鼠 Monoclonal BRD469 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), ICC, IF (cc), IF (p), IHC (fro), ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 小鼠 Monoclonal 4B6C12 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, Neut, StM 宿主: 兔 Monoclonal BRD2888R unconjugated Recombinant Antibody
anti-Bromodeoxyuridine (BrdU) antibody (PE)BrdU ICFC 宿主: 小鼠 Monoclonal Bu20a PE
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应用备注
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IC: 10 µg/mL, IHC: 10 µg/mL, IP: 25-100 µg/mL
Optimal conditions should be determined by the investigator. -
限制
- 仅限研究用
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浓度
- Lot specific
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缓冲液
- Protein G purified and supplied in PBS buffer, with no preservative.
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储存液
- Without preservative
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注意事项
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. -
储存条件
- -20 °C/-80 °C
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储存方法
- Aliquot and store at -20 °C for short term storage, -70 °C for long-term storage.
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: "Abnormal corneal epithelial maintenance in mice heterozygous for the micropinna microphthalmia mutation Mp." in: Experimental eye research, Vol. 149, pp. 26-39, (2017) (PubMed).
: "A Role for Androgens in Epithelial Proliferation and Formation of Glands in the Mouse Uterus." in: Endocrinology, Vol. 157, Issue 5, pp. 2116-28, (2017) (PubMed).
: "Timing of Smarcb1 and Nf2 inactivation determines schwannoma versus rhabdoid tumor development." in: Nature communications, Vol. 8, Issue 1, pp. 300, (2017) (PubMed).
: "Cell proliferation, movement and differentiation during maintenance of the adult mouse adrenal cortex." in: PLoS ONE, Vol. 8, Issue 12, pp. e81865, (2014) (PubMed).
: "Activation of the canonical bone morphogenetic protein (BMP) pathway during lung morphogenesis and adult lung tissue repair." in: PLoS ONE, Vol. 7, Issue 8, pp. e41460, (2012) (PubMed).
: "Geminin-deficient neural stem cells exhibit normal cell division and normal neurogenesis." in: PLoS ONE, Vol. 6, Issue 3, pp. e17736, (2011) (PubMed).
: "Identification of EphrinB1 expression in prostatic mesenchyme and a role for EphB-EphrinB signalling in prostate development." in: Differentiation; research in biological diversity, Vol. 80, Issue 2-3, pp. 89-98, (2010) (PubMed).
: "Quantitative analysis of postnatal neurogenesis and neuron number in the macaque monkey dentate gyrus." in: The European journal of neuroscience, Vol. 31, Issue 2, pp. 273-85, (2010) (PubMed).
: "A specialized vascular niche for adult neural stem cells." in: Cell stem cell, Vol. 3, Issue 3, pp. 279-88, (2008) (PubMed).
: "Sonic hedgehog regulates prostatic growth and epithelial differentiation." in: Developmental biology, Vol. 264, Issue 2, pp. 352-62, (2003) (PubMed).
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: "Abnormal corneal epithelial maintenance in mice heterozygous for the micropinna microphthalmia mutation Mp." in: Experimental eye research, Vol. 149, pp. 26-39, (2017) (PubMed).
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- BrdU (Bromodeoxyuridine (BrdU))
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别名
- BRDU
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物质类
- Chemical
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背景
- Bromodeoxyuridine is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical.
抗原
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