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MAPK11 抗体 (N-Term)

Name: MAPK11 抗体 (N-Term)
SKU: ABIN2798348
Availability: OutOfStock
Rating: 5 (1 reviews)

MAPK11 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Mitogen-Activated Protein Kinase 11 (MAPK11) (AA 1-30), (N-Term) antibody (ABIN2798348) (MAPK11 抗体 (N-Term))

1 image

(1 validation)

产品编号 ABIN2798348

发货至: 中国

Datasheet as PDF

抗原 See all MAPK11 抗体

MAPK11 (Mitogen-Activated Protein Kinase 11 (MAPK11))
抗原表位
15

15

15

14

8

7

5

4

3

2

2

2

1

1

1

1

1
AA 1-30, N-Term
适用
67

29

21

2

1

1
小鼠
宿主
80

5

3
兔
Compatible Secondaries
克隆类型
83

5
多克隆
标记
33

8

7

6

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1
This MAPK11 antibody is un-conjugated
应用范围
76

42

27

26

26

11

7

5

4

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

原理

Rabbit Anti-Mouse Mapk11 (N-term) Antibody

免疫原

This Mouse Mapk11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of mouse Mapk11.

亚型

Ig Fraction

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MAPK11 适用: 人 WB, ELISA, IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 11 (MAPK11) (AA 255-364) antibody
MAPK11 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 1F9 unconjugated

anti-Mitogen-Activated Protein Kinase 11 (MAPK11) (AA 1-364) antibody
MAPK11 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 11 (MAPK11) (AA 1-30), (N-Term) antibody
MAPK11 适用: 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB34635 unconjugated

anti-Mitogen-Activated Protein Kinase 11 (MAPK11) (AA 21-120) antibody
MAPK11 适用: 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 11 (MAPK11) (AA 310-362) antibody
MAPK11 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 11 (MAPK11) antibody
MAPK11 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 11 (MAPK11) (AA 1-364) antibody
MAPK11 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 11 (MAPK11) (AA 1-364) antibody
MAPK11 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 11 (MAPK11) (AA 251-363) antibody
MAPK11 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4H6H6 unconjugated

应用备注

Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:10-50Western blot analysis of lysate from mouse NIH/3T3 cell line, using Mouse Mapk11 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

限制

仅限研究用
生效 #100108 (Western Blotting)

by

Instituto de Parasitología y Biomedicina López-Neyra

No.

#100108

日期

2016.12.23

抗原

MAPK11

Lot Number

RAY8110713

Method validated

Western Blotting

Positive Control

primary mouse microglia

Negative Control

siRNA knock-down of MAPK11 in primary mouse microglia

Notes

Passed. ABIN2798348 specifically recognizes Mapk11 in primary mouse microglia lysates.

Validation Images

Primary mouse microglia were transfected with Mapk11 siRNA and Mapk11 was revealed using ABIN2798348 as described in the protocol section (lanes 1, 2, and 3). Lysates from cells transfected with scrambled siRNA (lane 4) and untransfected cells (lane 5) served as negative controls.
Full Methods
Primary Antibody

ABIN2798348

Secondary Antibody

anti-rabbit HRP-conjugated antibodies (DakoCytomation, P0448, lot 00055814)

Full Protocol

Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
Transfect cells with 0.05nmol mouse MAPK11 Silencer Select siRNA (ThermoFisher Scientific, Sample ID ASO10943, ASO1098J and ASO109CX, lot AMO04BXD) using Lipofectamine 3000 (Invitrogen) following the manufacturer´s instructions.
Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na₃VO₄, protease inhibitors (Sigma).
Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
Denature 5µg total protein for 5min at 95°C in 5µl Laemmli SDS sample buffer and subsequently separate them on a denaturing freshly cast 10% SDS-PAGE for 1h at 140V.
Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk ON at 4°C.
Incubation with primary rabbit anti-MAPK11 antibody (antibodies-online, ABIN2798348, lot RAY8110713) diluted 1:500 in TBST containing 5% milk for 1h at RT.
Wash membrane 5x 5min with TBST.
Incubation with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
Wash membrane 5x 5min with TBST and 3x 5min with TBS.
Reveal protein bands using ECL Prime Western Blotting Detection Reagent (GE Healthcare, RPN2232, lot 9547481) with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
Strip membranes RestoreTM Western Blot Stripping Buffer (Thermo Scientific, 21059, lot NA 164269) and subsequently incubate with a GAPDH loading control antibody (Sigma, G9545, lot 049K4787) diluted 1:10000 and with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000.
Wash and reveal the protein bands using self-made ECL Western Blotting Detection Reagent with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.

Experimental Notes

The MAPK11 antibody ABIN2798348 reveals a protein of the expected molecular weight of murine Mapk11 in lysates of primary mouse microglia. The protein band’s intensity decreases upon siRNA knockdown.
状态

Liquid

浓度

0.5 mg/mL

储存条件

4 °C,-20 °C

储存方法

2-8°C (short-term), -20°C (long-term)
抗原

MAPK11 (Mitogen-Activated Protein Kinase 11 (MAPK11))

别名

Mapk11 (MAPK11 产品)

别名

ATMPK11 antibody, F22L4.10 antibody, F22L4_10 antibody, MAP kinase 11 antibody, zgc:86905 antibody, P38B antibody, P38BETA2 antibody, PRKM11 antibody, SAPK2 antibody, SAPK2B antibody, p38-2 antibody, p38Beta antibody, P38b antibody, Prkm11 antibody, Sapk2 antibody, Sapk2b antibody, p38beta antibody, p38beta2 antibody, p38b antibody, sapk2 antibody, prkm11 antibody, sapk2b antibody, mitogen-activated protein kinase 11 antibody, MAP kinase 11 antibody, mapk11 antibody, LOC9328118 antibody, MPK11 antibody, MAPK11 antibody, Mapk11 antibody, PTRG_02565 antibody

背景

Target Description: Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2 (By similarity).
Gene Symbol: Mapk11

分子量

41397 Da

基因ID

19094

UniProt

Q9WUI1

途径

MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Response to Water Deprivation, Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Hepatitis C, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins