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PCNA 抗体 (C-Term)

Name: PCNA 抗体 (C-Term)
SKU: ABIN2792157
Availability: OutOfStock

PCNA 适用: 人, 小鼠, 大鼠, 犬, 斑马鱼, Cow WB, IHC 宿主: 兔 Polyclonal unconjugated

WB Suggested Anti-PCNA Antibody Titration: 1. (PCNA 抗体 (C-Term))

Sample Type: NT2 cells Red: Antibody Blue: DAPI Primary Dilution: 1ug/50ul antibody Secondary Antibody: Alexa goat anti-rabbit 594 Image Submitted by: Yuzhi Chen, University of Arkansas for Medical Sciences (PCNA 抗体 (C-Term))

IHC

IHC

Rabbit Anti-PCNA Antibody AVParaffin Embedded Tissue: Human Spleen Cellular Data: Spleen cells Antibody Concentration: 4. (PCNA 抗体 (C-Term))

IHC

6 images

(1 reference)

产品编号 ABIN2792157

发货至: 中国

Datasheet as PDF

抗原 See all PCNA 抗体

PCNA (Proliferating Cell Nuclear Antigen (PCNA))
抗原表位
16

16

16

15

11

11

8

6

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
207

149

148

54

28

27

21

19

18

18

17

13

10

10

6

6

5

5

4

4

4

3

3

3

2

2

2

2

2

1

1

1

1
人, 小鼠, 大鼠, 犬, 斑马鱼, Cow
宿主
129

91

3
兔
Compatible Secondaries
克隆类型
128

94

1
多克隆
标记
109

16

13

8

5

5

5

5

5

5

5

5

5

5

5

4

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This PCNA antibody is un-conjugated
应用范围
188

68

63

60

51

47

47

40

29

15

15

11

5

4

3

2

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

序列

LNFFTKATPL SSTVTLSMSA DVPLVVEYKI ADMGHLKYYL APKIEDEEGS

预测反应

Cow: 100%, Dog: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 91%

产品特性

This is a rabbit polyclonal antibody against PCNA. It was validated on Western Blot and immunohistochemistry.

纯化方法

Protein A purified

免疫原

The immunogen is a synthetic peptide directed towards the C terminal region of human PCNA

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anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 6-261) antibody
PCNA 适用: 人, 小鼠, 大鼠, Pig, 兔, 犬, 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

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anti-Proliferating Cell Nuclear Antigen (PCNA) antibody
PCNA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 261 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Liu, Meng, Li, Zhou, Zeng, He, Mrsny, Liu, Hu, Hu, Li: "Human umbilical cord mesenchymal stromal cells rescue mice from acetaminophen-induced acute liver failure." in: Cytotherapy, Vol. 16, Issue 9, pp. 1207-19, (2014) (PubMed).
抗原

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

别名

PCNA (PCNA 产品)

背景

PCNA is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Alias Symbols: MGC8367
Protein Interaction Partner: GADD45A, CDKN1A, HUWE1, POLH, PCNA, MSH3, FUS, UBC, TMEM218, CHFR, DTL, POLE, POLD1, MCM7, MCM4, LIG1, MSH6, FEN1, FBXO18, ZRANB3, CHTF18, DNMT1, RAD18, SIVA1, KIAA0101, TDG, SUMO2, SUMO3, RPA1, RFC5, RFC4, RFC3, RFC2, RFC1, MDM2, TRIM25, USP18, ISG15, MA
Protein Size: 261

分子量

29 kDa

基因ID

5111

NCBI登录号

NM_002592, NP_002583

UniProt

P12004

途径

Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy