Plakophilin 2 抗体 (N-Term)
(1 validation)-
- 抗原 See all Plakophilin 2 (PKP2) 抗体
- Plakophilin 2 (PKP2)
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抗原表位
- N-Term
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适用
- 人, 大鼠, 小鼠, 犬, Cow, 马
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Plakophilin 2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 序列
- HPLRRLEISP DSSPERAHYT HSDYQYSQRS QAGHTLHHQE SRRAALLVPP
- 预测反应
- Cow: 77%, Dog: 79%, Horse: 86%, Human: 100%, Mouse: 86%, Rat: 77%
- 产品特性
- This is a rabbit polyclonal antibody against PKP2. It was validated on Western Blot using a cell lysate as a positive control.
- 纯化方法
- Affinity Purified
- 免疫原
- The immunogen is a synthetic peptide directed towards the N terminal region of human PKP2
- Top Product
- Discover our top product PKP2 Primary Antibody
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anti-Plakophilin 2 (PKP2) (AA 801-881) antibody
PKP2 适用: 人 ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Plakophilin 2 (PKP2) (C-Term) antibodyPKP2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Plakophilin 2 (PKP2) (AA 61-183) antibodyPKP2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Plakophilin 2 (PKP2) (C-Term) antibodyPKP2 适用: 人, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Plakophilin 2 (PKP2) (AA 1-350) antibodyPKP2 适用: 人, 小鼠 WB, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 8H6 unconjugated
anti-Plakophilin 2 (PKP2) (N-Term) antibodyPKP2 适用: 人, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Plakophilin 2 (PKP2) (AA 125-139) antibodyVerified PKP2 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Plakophilin 2 (PKP2) (AA 571-849) antibodyPKP2 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Plakophilin 2 (PKP2) antibodyPKP2 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Plakophilin 2 (PKP2) (AA 143-192) antibodyPKP2 适用: 人, 小鼠, 犬, Cow, 马 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
- 说明
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Antigen size: 837 AA
- 限制
- 仅限研究用
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- by
- Gencardio (IDIBGI)
- No.
- #103654
- 日期
- 2019.03.13
- 抗原
- PKP2
- Lot Number
- QC27639-43357
- Method validated
- Western Blotting
- Positive Control
HL-1 cell extracts (from AT-1 mouse atrial cardiomyocyte tumor lineage)
Extracts from HEK293 cells transfected with human PKP2-FLAG expressing plasmid
Extract of mouse heart tissue (endogenous PKP2 expected molecular weight 88KDa)
Extract of human heart tissue (endogenous PKP2 expected molecular weight 91KDa)
- Negative Control
Extracts from untransfected HEK293 cells
- Notes
ABIN2786784 specifically recognizes PKP2 in cell extracts from HEK-293 overexpressing human PKP2 protein and endogenous PKP2 in human heart tissue but not endogenous mouse PKP2 neither in HL-1 cells nor mouse tissue.
- Primary Antibody
- ABIN2786784
- Secondary Antibody
- mouse anti-FLAG-tag antibody (Sigma, monoclonal anti-flag, F316, lot SLBT7654)
- Full Protocol
- Grow HL-1 cells in Claycomb medium (Sigma, 51800C, lot SLBX8700) supplemented with 10% FBS (Gibco, 10270-106, lot 42G5178K), 1% Penicillin-Streptomycin (Sigma, P4333, lot 048M4874V) and 1% Glutamax (Gibco, 35050-061, 2037045), at 37°C and 5% CO2 in 2ml on a 6 well plate to 95% confluency.
- Grow HEK293 cells in DMEM/High Modified medium (GE Life Science, SH30285.01, lot AC14565263) supplemented with 10% FBS, 1% Penicillin-Streptomycin and 1% Glutamax (Gibco, 35050-061, 2037045), at 37°C and 5% CO2 in 2ml on a 6 well plate to 95% confluency.
- Transfect HEK293 cells with PKP2a plasmid (p915 PKP2a-pFLAG-cmv5a; provided by Dr. Green KJ) using Lipofectamin 2000 (Invitrogen, 11668-019, lot 2022713) following the manufacturer´s instructions.
- Lyse approximately 1.2x106 cells per well in 200µl 1% SDS (Sigma, 75746, lot STBG4187V).
- Homogenate whole heart of transgenic and wt heart mouse tissue with M-Per reagent (Thermo, 78501, lot NC168900) and mechanical disgregation using a Dounce homogenizer.
- Incubate for the lysates from 15min at 95°C.
- Vortex for 15min.
- Determine total protein content of the lysates using Pierce BCA Protein Assay Kit (Thermo Scientific, 23227, lot TC263613).
- Denature 15µg (HEK293 cell lysates), 106µg (HL-1 cell lysates), and 100µg (mouse heart tissue lysates) of total protein for 5min at 95°C in 45µl using 6x Laemmli SDS sample buffer and subsequently separate them on a 8% acrylamide gel in an electrophoresis chamber for 30min at 80V and 1h at 120V.
- Transfer proteins onto Amersham Hybond P 0.45 PVDF blotting membrane (GE Life Science, 10600023, lot A16954274) for 2h at 80V.
- Block the membrane with 5% NFM in PBST for 1h at RT.
- Incubation with primary
- rabbit anti-Plakophilin 2 (PKP2) (N-Term) antibody (antibodies-online, ABIN2786784, lot QC27639-43357) diluted 1:800 in 5% NFM in PBS ON at 4°C or
- mouse anti-FLAG-tag antibody (Sigma, monoclonal anti-flag, F316, lot SLBT7654) diluted 1:x in 5% NFM in PBS ON at 4°C.
- Wash membrane 2x for 5’ with PBST and 2x for 5min with PBS.
- Block the membrane with 5% NFM in PBST for 1h at RT.
- Incubation with secondary anti-rabbit (Jackson ImmunoResearch, 111-035-003, lot # 135002) diluted 1:10000 in 5% NFM in PBS for 1h at RT.
- Wash membrane 2x for 5min with PBST and 2x for 5min with PBS.
- Reveal protein bands using Perce ECL Western (Thermo Scientific, 32106, TE265714) with Amersham Hyperfilm ECL (GE Healthcare Limited, 28906835, 65901).
- Experimental Notes
HEK293 cells with no endogenous PKP2 expression have been transfected with human FLAG-tagged PKP2 as a positive control. HL-1 cells express endogenous mouse PKP2.
生效 #103654 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Western blot of lysates from HEK293 cells transfected with FLAG-tagged PKP2 (1), non-transfected HEK293 cells (2), non-transfected HL1 cells (3) HEK293 cells transfected with ABIN2786784 or an anti-FLAG tag antibody. In addition, lysates from human (4) and mouse heart tissue (5, 6) were incubated with ABIN2786784.]()
Western blot of lysates from HEK293 cells transfected with FLAG-tagged PKP2 (1), non-transfected HEK293 cells (2), non-transfected HL1 cells (3) HEK293 cells transfected with ABIN2786784 or an anti-FLAG tag antibody. In addition, lysates from human (4) and mouse heart tissue (5, 6) were incubated with ABIN2786784.
Full Methods -
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- 抗原
- Plakophilin 2 (PKP2)
- 别名
- PKP2 (PKP2 产品)
- 别名
- ARVD9 antibody, 1200008D14Rik antibody, 1200012P04Rik antibody, AA516617 antibody, Pkp2l antibody, plakophilin 2 antibody, PKP2 antibody, Pkp2 antibody
- 背景
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This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the
Alias Symbols: ARVD9
Protein Interaction Partner: NDEL1, MTUS2, CEP250, DROSHA, BMI1, EED, RNF2, YWHAB, CLK2, YWHAE, WHSC1, UBC, SMAD9, YWHAQ, CUL3, MARK3, POLR3A, YWHAG, PKP2, JUP, KRT18, KRT5, DSG2, DSG1, DSP, DSC2, DSC1, CTNNB1, GTF2B, SFN,
Protein Size: 837 - 分子量
- 93 kDa
- 基因ID
- 5318
- NCBI登录号
- NM_001005242, NP_001005242
- UniProt
- A0AV37
- 途径
- Cell-Cell Junction Organization, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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