ETF1 抗体 (N-Term)
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- 抗原 See all ETF1 抗体
- ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))
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抗原表位
- N-Term
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适用
- 人, 小鼠, 大鼠, 豚鼠, 斑马鱼, 马, Cow, 犬, 兔, Saccharomyces cerevisiae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ETF1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 序列
- ISLIIPPKDQ ISRVAKMLAD EFGTASNIKS RVNRLSVLGA ITSVQQRLKL
- 预测反应
- Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 100%
- 产品特性
- This is a rabbit polyclonal antibody against ETF1. It was validated on Western Blot using a cell lysate as a positive control.
- 纯化方法
- Affinity Purified
- 免疫原
- The immunogen is a synthetic peptide directed towards the N terminal region of human ETF1
- Top Product
- Discover our top product ETF1 Primary Antibody
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anti-Eukaryotic Translation Termination Factor 1 (ETF1) (AA 338-437) antibody
ETF1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H4 unconjugated
anti-Eukaryotic Translation Termination Factor 1 (ETF1) antibodyETF1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Termination Factor 1 (ETF1) antibodyETF1 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 2E3 unconjugated
anti-Eukaryotic Translation Termination Factor 1 (ETF1) (AA 278-437) antibodyETF1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Termination Factor 1 (ETF1) antibodyETF1 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Termination Factor 1 (ETF1) (AA 35-84) antibodyETF1 适用: 人, 小鼠, 大鼠, 豚鼠, 斑马鱼, 马, Cow, 犬, 兔, 非洲爪蟾, Drosophila melanogaster, Hamster, Bat, 猴, 小鸡, Pig WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
- 说明
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Antigen size: 437 AA
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- 抗原
- ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))
- 别名
- ETF1 (ETF1 产品)
- 背景
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Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA, however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).
Alias Symbols: D5S1995, ERF, ERF1, MGC111066, RF1, SUP45L1, TB3-1
Protein Interaction Partner: KEAP1, UBC, RTF1, NUDC, VPS26A, TSN, CORO1C, CORO1B, HECW2, ALB, UPF1, LIG4, GSPT1, PPP2CA, GSPT2, PABPC1,
Protein Size: 437 - 分子量
- 49 kDa
- 基因ID
- 2107
- NCBI登录号
- NM_004730, NP_004721
- UniProt
- P62495
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