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CCDC42 抗体 (Middle Region)

CCDC42 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN2785068
发货至: 中国
  • 抗原 See all CCDC42 抗体
    CCDC42 (Coiled-Coil Domain Containing 42 (CCDC42))
    抗原表位
    • 15
    • 8
    • 7
    • 6
    • 6
    • 6
    • 4
    • 1
    Middle Region
    适用
    人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔
    宿主
    • 38
    克隆类型
    • 38
    多克隆
    标记
    • 8
    • 5
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CCDC42 antibody is un-conjugated
    应用范围
    • 32
    • 22
    • 14
    • 13
    • 13
    • 4
    • 3
    Western Blotting (WB)
    序列
    ENSEFEEIHE VIARYKTLVS MHHDLMQSAQ EGQEKIEHAK ARLARYMEEK
    预测反应
    Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 93%
    产品特性
    This is a rabbit polyclonal antibody against Ccdc42. It was validated on Western Blot.
    纯化方法
    Affinity Purified
    免疫原
    The immunogen is a synthetic peptide directed towards the middle region of Rat Ccdc42
    Top Product
    Discover our top product CCDC42 Primary Antibody
  • 应用备注
    Optimal working dilutions should be determined experimentally by the investigator.
    说明

    Antigen size: 316 AA

    限制
    仅限研究用
  • 生效 #103398 (Immunocytochemistry)
    '独立验证'标志
    by
    Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University of Göttingen
    No.
    #103398
    日期
    2018.10.12
    抗原
    CCDC42
    Lot Number
    QC28887-41199
    Method validated
    Immunocytochemistry
    Positive Control
    NIH/3T3 cells transfected with Cddc42-mCherry expression vector
    Negative Control
    Non-transfected NIH/3T3 cells
    Notes

    Passed. ABIN2785068 specifically detects Ccdc42-Cherry when exogenously expressed. Background staining is low.

    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN2785068
    Secondary Antibody
    goat anti-rabbit 488 antibody (MoBiotec, MFP-A1008, lot 2806F058)
    Full Protocol
    • Grow NIH/3T3 cells (ATCC, CRL-1658) in DMEM+GlutaMAX (Gibco, 10567-014, lot 1922818) supplemented with fetal bovine serum (Gibco, 10500-064, lot 08FO477K) and Pen/Strep (Gibco, 15140-122, lot 1924797) at 37°C and 5% CO2 to 70% confluency.
    • Transfect cells with Ccdc42 tagged mCherry (Ccdc42-Cherry) expression vector using EndofectinMax (GeneCopoeia) following the manufacturer´s instructions.
    • Grow cells for 24h.
    • Fix cells in methanol for 10min at -20°C followed by incubation in 0.3% Triton X-100 for 10min at 4°C.
    • Block cells in PBS containing 1% bovine serum albumin and 0.5% Tween-20 (PBT) for 1h at RT.
    • Incubate cells with primary rabbit anti-Ccdc42 antibody (antibodies-online, ABIN2785068, QC28887-41199) diluted 1:100 in PBS for 1h at 37°C.
    • Wash cells 3x 5min with 1x PBS.
    • Incubate cells with secondary goat anti-rabbit 488 antibody (MoBiotec, MFP-A1008, lot 2806F058) diluted 1:300 for 1h at 37°C in PBS.
    • DNA was counterstained with DAPI (4’, 6-Diamidino-2-phenylindole; Sigma, D-9542).
    • Image acquisition on Zeiss LSM 510 confocal microscope and processing using Adobe Photoshop 5.0.
    Experimental Notes
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -20 °C
    储存方法
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Wu, Yu, Liu, Wang, Huang, Lu, Chen, Li, Liu: "Essential Role of CFAP53 in Sperm Flagellum Biogenesis." in: Frontiers in cell and developmental biology, Vol. 9, pp. 676910, (2021) (PubMed).

    Tapia Contreras, Hoyer-Fender: "CCDC42 Localizes to Manchette, HTCA and Tail and Interacts With ODF1 and ODF2 in the Formation of the Male Germ Cell Cytoskeleton." in: Frontiers in cell and developmental biology, Vol. 7, pp. 151 (PubMed).

  • 抗原
    CCDC42 (Coiled-Coil Domain Containing 42 (CCDC42))
    别名
    Ccdc42 (CCDC42 产品)
    别名
    RGD1565925 antibody, CCDC42A antibody, A530001H01Rik antibody, coiled-coil domain containing 42 antibody, Ccdc42 antibody, CCDC42 antibody
    背景
    The function of this protein remains unknown.
    Alias Symbols: RGD1565925
    Protein Size: 316
    分子量
    34 kDa
    基因ID
    303234
    NCBI登录号
    NM_001107009, NP_001100479
    UniProt
    D3ZAZ8
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