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ELF1 抗体 (N-Term)

Name: ELF1 抗体 (N-Term)
SKU: ABIN2779831
Availability: OutOfStock
Rating: 5 (1 reviews)

ELF1 适用: 人, 小鼠, 兔, 大鼠, 马, 犬 WB 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (N-Term) antibody (ABIN2779831) (ELF1 抗体 (N-Term))

WB Suggested Anti-ELF1 Antibody Titration: 0. (ELF1 抗体 (N-Term))

WB Suggested Anti-ELF1 antibody Titration: 1 ug/mL Sample Type: Human MCF7 (ELF1 抗体 (N-Term))

WB Suggested Anti-ELF1 antibody Titration: 1 ug/mL Sample Type: Human Hela (ELF1 抗体 (N-Term))

4 images

(1 validation)

产品编号 ABIN2779831

发货至: 中国

Datasheet as PDF

抗原 See all ELF1 抗体

ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))
抗原表位
15

5

4

4

1

1

1

1

1

1

1

1
N-Term
适用
44

6

6

5

3

2

2

2

1

1

1

1
人, 小鼠, 兔, 大鼠, 马, 犬
宿主
40

3

1
兔
Compatible Secondaries
克隆类型
43

1
多克隆
标记
24

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This ELF1 antibody is un-conjugated
应用范围
35

13

13

11

11

6

6

3

2

1

1

1
Western Blotting (WB)

序列

DDIVAPITHV SVTLDGIPEV METQQVQETN ADSPGASSPE QRKRKKGRKT

预测反应

Dog: 80%, Horse: 80%, Human: 87%, Mouse: 100%, Rabbit: 87%, Rat: 88%

产品特性

This is a rabbit polyclonal antibody against ELF1. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the N terminal region of mouse ELF1

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ELF1 适用: 人, 小鼠, 兔, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 612 AA

限制

仅限研究用
生效 #104222 (Western Blotting)

by

Dittmann Lab, Microbiology Department, NYU Langone Health, NYU School of Medicine

No.

#104222

日期

2019.10.28

抗原

ELF1

Lot Number

66160414

Method validated

Western Blotting

Positive Control

A549 (endogenous ELF1), ELF1 recombinant protein

Negative Control

Notes

Passed. ABIN2779831 specifically recognizes endogenous ELF1 in A549 cell lysates and recombinant human ELF1.

Validation Images

Detection of human ELF1 by western blot using ABIN2779831 diluted 1:500. Samples: whole cell lysate from A549 cells and human ELF1 recombinant protein. Expected molecular weight: approximately 67kDa.
Full Methods
Primary Antibody

ABIN2779831

Secondary Antibody

goat anti-rabbit HRP-conjugated antibody (Invitrogen, G21234)

Full Protocol

Grow A549 cells (ATCC, CCL-185) in DMEM medium (Corning, 10-013-CV) supplemented with 10% FBS (Atlanta biologicals, S11150) and Penicillin-Streptomycin (Corning, 30-002-CI), at 37°C and 5% CO₂ to 3x10⁴ cells/cm² in 2ml on a 6 well plate (Costar, 3516).
Wash cells once with 1x PBS treat with trypsin (Corning, 25-053-CI).
Take cells up in growth medium.
Wash cells once with 1x PBS.
Resuspend approximately 2.05x10⁵cells/well in 50-100µl 1x LDS sample buffer (Life Technologies, B0007). As positive control, take 20µg recombinant ELF1 (Abnova, H00001997-P01) in 1x LDS sample buffer.
Denature samples for 3min at 95°C and subsequently keep them on ice.
Separate samples on a Bolt 4-12% Bis-Tris Plus Gel (Invitrogen, NW04122) in an electrophoresis chamber (Mini Gel Tank, Invitrogen, A25977) for 30min at 80V and then for 60min at 120V.
Transfer proteins onto nitrocellulose membrane (Invitrogen, IB23002) using an iBlot 2 Gel Transfer Device (Invitrogen).
Block the membrane with TBS containing 0.05% Tween (TBST) containing 5% skim milk for 1h at RT.
Incubation with primary
o rabbit anti-ELF1 antibody (antibodies-online, ABIN2779831, 0066160414) diluted 1:500 in TBST containing 5% skim milk ON at 4°C.
loading control mouse anti-beta actin antibody (Invitrogen, MA5-15739) diluted 1:5000 in TBST containing 0.5% skim milk ON at 4°C.
Wash membrane 3x for 10min with TBST.
Incubation with secondary
goat anti-rabbit HRP-conjugated antibody (Invitrogen, G21234) diluted 1:10000 in TBST containing 0.5% skim milk for 1h at RT.
goat anti-mouse HRP-conjugated antibody (Invitrogen, G21040) diluted 1:10000 in TBST containing 0.5% skim milk for 1h at RT.
Wash membrane 3x for 10min with TBST.
Reveal protein bands using SuperSignal West Dura Extended Duration Substrate (ThermoFisher Scientific, 34075) and a ChemiDoc MP Imaging System (Bio-Rad).

Experimental Notes

ABIN2779831 reveals a protein with an apparent molecular weight of the expected MW of 67kDa for ELF1 and some extraneous bands of lower intensity. ABIN2779831 also reveals a recombinant GST-tagged protein at the expected molecular weight (theoretical MW 93.9kDa).
Other ELF1-antibody dilutions were tested, but a dilution of 1:5000 was found optimal in terms of minimal background and strength of signal.
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
抗原

ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))

别名

ELF1 (ELF1 产品)

别名

zef1 antibody, fb05d07 antibody, wu:fb05d07 antibody, Elf-1 antibody, Sts1 antibody, mElf-1 antibody, p70 antibody, xelf1 antibody, elf-1 antibody, E74 like ETS transcription factor 1 antibody, E74-like ETS transcription factor 1 antibody, E74-like factor 1 antibody, E74 like ETS transcription factor 1 L homeolog antibody, E74 like ETS transcription factor 1 S homeolog antibody, ELF1 antibody, elf1 antibody, Elf1 antibody, elf1.L antibody, elf1.S antibody

背景

Elf1 belongs to the ETS family. Elf1 is a transcription factor that activates the LYN and BLK promoters. Elf1 may interact with other transcription factors in order to regulate specific genes. Elf1 can bind to the underphosphorylated form of RB.
Alias Symbols: p70, Sts1, Elf-1, mElf-1
Protein Interaction Partner: Ephb4, Dmd, Jak2, Foxp3,
Protein Size: 612

分子量

67 kDa

基因ID

13709

NCBI登录号

NM_007920, NP_031946

UniProt

Q60775