ELF1 抗体 (N-Term)
(1 validation)-
- 抗原 See all ELF1 抗体
- ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))
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抗原表位
- N-Term
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适用
- 人, 小鼠, 兔, 大鼠, 马, 犬
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ELF1 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 序列
- DDIVAPITHV SVTLDGIPEV METQQVQETN ADSPGASSPE QRKRKKGRKT
- 预测反应
- Dog: 80%, Horse: 80%, Human: 87%, Mouse: 100%, Rabbit: 87%, Rat: 88%
- 产品特性
- This is a rabbit polyclonal antibody against ELF1. It was validated on Western Blot using a cell lysate as a positive control.
- 纯化方法
- Affinity Purified
- 免疫原
- The immunogen is a synthetic peptide directed towards the N terminal region of mouse ELF1
- Top Product
- Discover our top product ELF1 Primary Antibody
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anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (C-Term) antibody
Verified ELF1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (C-Term) antibodyELF1 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 1-210) antibodyELF1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 421-520) antibodyELF1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B7 unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 1-619) antibodyELF1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 142-191) antibodyELF1 适用: 人, 兔, 大鼠, 马, 犬, Cow, 豚鼠, Bat, Hamster, 猴, 非洲爪蟾 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (C-Term) antibodyELF1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 1-619) antibodyELF1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 540-589) antibodyELF1 适用: 人, 兔, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 131-180) antibodyELF1 适用: 人, 小鼠, 兔, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
- 说明
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Antigen size: 612 AA
- 限制
- 仅限研究用
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- by
- Dittmann Lab, Microbiology Department, NYU Langone Health, NYU School of Medicine
- No.
- #104222
- 日期
- 2019.10.28
- 抗原
- ELF1
- Lot Number
- 66160414
- Method validated
- Western Blotting
- Positive Control
A549 (endogenous ELF1), ELF1 recombinant protein
- Negative Control
- Notes
Passed. ABIN2779831 specifically recognizes endogenous ELF1 in A549 cell lysates and recombinant human ELF1.
- Primary Antibody
- ABIN2779831
- Secondary Antibody
- goat anti-rabbit HRP-conjugated antibody (Invitrogen, G21234)
- Full Protocol
- Grow A549 cells (ATCC, CCL-185) in DMEM medium (Corning, 10-013-CV) supplemented with 10% FBS (Atlanta biologicals, S11150) and Penicillin-Streptomycin (Corning, 30-002-CI), at 37°C and 5% CO2 to 3x104 cells/cm2 in 2ml on a 6 well plate (Costar, 3516).
- Wash cells once with 1x PBS treat with trypsin (Corning, 25-053-CI).
- Take cells up in growth medium.
- Wash cells once with 1x PBS.
- Resuspend approximately 2.05x105cells/well in 50-100µl 1x LDS sample buffer (Life Technologies, B0007). As positive control, take 20µg recombinant ELF1 (Abnova, H00001997-P01) in 1x LDS sample buffer.
- Denature samples for 3min at 95°C and subsequently keep them on ice.
- Separate samples on a Bolt 4-12% Bis-Tris Plus Gel (Invitrogen, NW04122) in an electrophoresis chamber (Mini Gel Tank, Invitrogen, A25977) for 30min at 80V and then for 60min at 120V.
- Transfer proteins onto nitrocellulose membrane (Invitrogen, IB23002) using an iBlot 2 Gel Transfer Device (Invitrogen).
- Block the membrane with TBS containing 0.05% Tween (TBST) containing 5% skim milk for 1h at RT.
- Incubation with primary
- o rabbit anti-ELF1 antibody (antibodies-online, ABIN2779831, 0066160414) diluted 1:500 in TBST containing 5% skim milk ON at 4°C.
- loading control mouse anti-beta actin antibody (Invitrogen, MA5-15739) diluted 1:5000 in TBST containing 0.5% skim milk ON at 4°C.
- Wash membrane 3x for 10min with TBST.
- Incubation with secondary
- goat anti-rabbit HRP-conjugated antibody (Invitrogen, G21234) diluted 1:10000 in TBST containing 0.5% skim milk for 1h at RT.
- goat anti-mouse HRP-conjugated antibody (Invitrogen, G21040) diluted 1:10000 in TBST containing 0.5% skim milk for 1h at RT.
- Wash membrane 3x for 10min with TBST.
- Reveal protein bands using SuperSignal West Dura Extended Duration Substrate (ThermoFisher Scientific, 34075) and a ChemiDoc MP Imaging System (Bio-Rad).
- Experimental Notes
ABIN2779831 reveals a protein with an apparent molecular weight of the expected MW of 67kDa for ELF1 and some extraneous bands of lower intensity. ABIN2779831 also reveals a recombinant GST-tagged protein at the expected molecular weight (theoretical MW 93.9kDa).
Other ELF1-antibody dilutions were tested, but a dilution of 1:5000 was found optimal in terms of minimal background and strength of signal.
生效 #104222 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Detection of human ELF1 by western blot using ABIN2779831 diluted 1:500. Samples: whole cell lysate from A549 cells and human ELF1 recombinant protein. Expected molecular weight: approximately 67kDa.]()
Detection of human ELF1 by western blot using ABIN2779831 diluted 1:500. Samples: whole cell lysate from A549 cells and human ELF1 recombinant protein. Expected molecular weight: approximately 67kDa.
Full Methods -
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- 抗原
- ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))
- 别名
- ELF1 (ELF1 产品)
- 别名
- zef1 antibody, fb05d07 antibody, wu:fb05d07 antibody, Elf-1 antibody, Sts1 antibody, mElf-1 antibody, p70 antibody, xelf1 antibody, elf-1 antibody, E74 like ETS transcription factor 1 antibody, E74-like ETS transcription factor 1 antibody, E74-like factor 1 antibody, E74 like ETS transcription factor 1 L homeolog antibody, E74 like ETS transcription factor 1 S homeolog antibody, ELF1 antibody, elf1 antibody, Elf1 antibody, elf1.L antibody, elf1.S antibody
- 背景
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Elf1 belongs to the ETS family. Elf1 is a transcription factor that activates the LYN and BLK promoters. Elf1 may interact with other transcription factors in order to regulate specific genes. Elf1 can bind to the underphosphorylated form of RB.
Alias Symbols: p70, Sts1, Elf-1, mElf-1
Protein Interaction Partner: Ephb4, Dmd, Jak2, Foxp3,
Protein Size: 612 - 分子量
- 67 kDa
- 基因ID
- 13709
- NCBI登录号
- NM_007920, NP_031946
- UniProt
- Q60775
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