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beta Actin 抗体 (Middle Region)

ACTB 适用: 人, 小鼠, 大鼠, Pig, 绵羊, Cow, 斑马鱼, 马, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated

WB Suggested Anti-ACTB Antibody Positive Control: Lane 1: 341 µg mouse 3T3 ECM extract + blocking peptide, Lane 2: 041 µg mouse 3T3 ECM extract, Lane 3: 041 µg Echinococcus granulosus extract Primary Antibody Dilution: 1:000Secondary Antibody: Anti-rabbit-HRP Secondry Antibody Dilution: 1:00,000Submitted by: Anonymous (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Tissue: HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 µg/mL Peptide Concentration: 5. (beta Actin 抗体 (Middle Region))

Sample Type: NT2 cells Red: Antibody Blue: DAPI Primary Dilution: 1ug/50ul antibody Secondary Antibody: Alexa goat anti-rabbit 594 Image Submitted by: Yuzhi Chen, University of Arkansas for Medical Sciences (beta Actin 抗体 (Middle Region))

Sample type: 1-8. (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: Human Adult Placenta Antibody Dilution: 1. (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTBM Sample Type: Fetal Lung lysates Antibody Dilution: 1ug/ml (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: MCF7 Antibody Dilution: 1. (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: HepG2 Whole Cell lysates Antibody Dilution: 1. (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: Human Fetal Lung Antibody Dilution: 1. (beta Actin 抗体 (Middle Region))

WB Suggested Anti-ACTB Antibody Titration: 0. (beta Actin 抗体 (Middle Region))

Host: Mouse Target Name: ACTG1 Sample Tissue: Mouse Testis Antibody Dilution: 1ug/ml (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Tissue: Mouse Heart Antibody Dilution: 1ug/ml (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: Hela Antibody Dilution: 1. (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Tissue: Human HepG2 Antibody Dilution: 1. (beta Actin 抗体 (Middle Region))

Sample Type : Human brain stem cells Primary Antibody Dilution : 1:500 Secondary Antibody : Goat anti-rabbit Alexa-Fluor 594 Secondary Antibody Dilution : 1:1000 Color/Signal Descriptions : ACTB: Red DAPI:Blue Gene Name : ACTB Submitted by : Dr. (beta Actin 抗体 (Middle Region))

Sample type: 1-8. (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: HepG2 Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1ug/ml Peptide Concentration: 5. (beta Actin 抗体 (Middle Region))

IHC Information: Paraffin embedded breast tissue, tested with an antibody dilution of 5 ug/ml. (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: Jurkat Antibody Dilution: 1. (beta Actin 抗体 (Middle Region))

Rabbit Anti-ACTB Antibody Formalin Fixed Paraffin Embedded Tissue: Human Appendix (Colon) Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0. (beta Actin 抗体 (Middle Region))

20 images

(2 references)

产品编号 ABIN501930

发货至: 中国

Datasheet as PDF

抗原 See all beta Actin (ACTB) 抗体

beta Actin (ACTB) (Actin, beta (ACTB))
抗原表位
40

18

16

6

6

5

5

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Middle Region
适用
193

137

119

65

52

44

37

36

32

25

20

20

12

10

9

8

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠, Pig, 绵羊, Cow, 斑马鱼, 马, 山羊
宿主
115

94

5

1

1
兔
Compatible Secondaries
克隆类型
114

100
多克隆
标记
130

14

13

11

4

4

4

4

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is un-conjugated
应用范围
176

78

60

51

35

30

27

22

13

13

6

6

6

5

5

2

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

序列

TMKIKIIAPP ERKYSVWIGG SILASLSTFQ QMWISKQEYD ESGPSIVHRK

预测反应

Cow: 100%, Goat: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

产品特性

This is a rabbit polyclonal antibody against ACTB. It was validated on Western Blot.

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the middle region of Human ACTB

Top Product

Discover our top product ACTB Primary Antibody
anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人, 小鼠, 大鼠, 兔, Pig, Cow WB, ELISA, IHC (p), FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人 WB, IF, IP 宿主: 小鼠 Monoclonal unconjugated

anti-Actin, beta (ACTB) (AA 359-368) antibody
ACTB 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 2-16) antibody
ACTB 适用: 小鼠, 大鼠, 小鸡, 斑马鱼 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB 适用: 人, 小鼠, 大鼠, Pig, Cow WB, IHC (p) 宿主: 小鼠 Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 小鸡, 兔, Pig, Cow, Hamster, 山羊, 鱼 WB, ELISA 宿主: 小鼠 Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), FACS, DNA Mic 宿主: 小鼠 Monoclonal 137CT26-1-1 unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 375 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and may contain up to 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Phungphong, Kijtawornrat, Wattanapermpool, Bupha-Intr: "Regular exercise modulates cardiac mast cell activation in ovariectomized rats." in: The journal of physiological sciences : JPS, Vol. 66, Issue 2, pp. 165-73, (2016) (PubMed).

Rinne, Clements, Lamme, Duijf, Bolat, Meijer, Scheffer, Rosser, Tan, McGrath, Schalkwijk, Brunner, Zhou, van Bokhoven: "A novel translation re-initiation mechanism for the p63 gene revealed by amino-terminal truncating mutations in Rapp-Hodgkin/Hay-Wells-like syndromes." in: Human molecular genetics, Vol. 17, Issue 13, pp. 1968-77, (2008) (PubMed).
抗原

beta Actin (ACTB) (Actin, beta (ACTB))

别名

ACTB (ACTB 产品)

别名

BRWS1 antibody, PS1TP5BP1 antibody, ACTA1 antibody, Actx antibody, E430023M04Rik antibody, beta-actin antibody, ps1tp5bp1 antibody, act-2 antibody, actb antibody, B-actin antibody, LOC100174883 antibody, LOC100304816 antibody, A antibody, ACT5C antibody, Ac5C antibody, Act antibody, Act-5C antibody, Act5 antibody, Act5c antibody, Actin antibody, Actin/BAP47 antibody, Actin5C antibody, BAP47 antibody, Bap47 antibody, CG4027 antibody, Dmel\\CG4027 antibody, M32055 antibody, T11 antibody, act antibody, act 5C antibody, act5C antibody, actin antibody, actin5C antibody, anon-EST:fe2D2 antibody, beta-actin/Bap47 antibody, cyt5C antibody, l(1)G0009 antibody, l(1)G0010 antibody, l(1)G0025 antibody, l(1)G0079 antibody, l(1)G0117 antibody, l(1)G0177 antibody, l(1)G0245 antibody, l(1)G0330 antibody, l(1)G0420 antibody, l(1)G0486 antibody, Bact antibody, ACT-5 antibody, ACTB antibody, B-ACTZF antibody, actba antibody, bact antibody, bactin1 antibody, bactzf antibody, wu:fd18f05 antibody, A26C1B antibody, POTE2alpha antibody, POTEACTIN antibody, Beta-actin antibody, actin beta antibody, actin, beta antibody, cytoplasmic actin OlCA1 antibody, beta-actin antibody, Actin 5C antibody, actin gamma 1 antibody, actin, beta 1 antibody, actin, beta L homeolog antibody, beta actin antibody, POTE ankyrin domain family member F antibody, actin, beta like 2 antibody, actin, cytoplasmic 1 antibody, ACTB antibody, Actb antibody, actb antibody, LOC100174883 antibody, LOC100304816 antibody, Act5C antibody, ACTG1 antibody, actb1 antibody, actb.L antibody, LOC100136352 antibody, POTEF antibody, ACTBL2 antibody, LOC109073280 antibody

背景

This protein is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Alias Symbols: BRWS1, PS1TP5BP1
Protein Interaction Partner: FUS, DSTN, CAP2, CFL2, CFL1, ACTG1, ACTB, PA2G4, EHHADH, WDYHV1, HDAC6, ISG15, TP53, CEP250, TUBGCP2, TUBGCP3, VCP, UBC, TUBG1, SUMO2, LGALS3BP, AMOT, NEDD1, HAUS1, IVNS1ABP, STAU1, MDM2, CORO7, LGR4, ASB12, ASB9, ZBTB1, ERG, RPA3, RPA2, RPA1, CDKN2A, PAR
Protein Size: 375

分子量

42 kDa

基因ID

60

NCBI登录号

NM_001101, NP_001092

UniProt

P60709

途径

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction

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